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DetectaGene™ Green CMFDG lacZ Gene Expression Kit

Catalog number: D2920

Molecular Probes™  Related applications: Cellular Imaging | Flow Cytometry | Protein Assays and Analysis

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Description

The DetectaGene™ Green lacZ Gene Expression Kit includes a fluorescein-based β-galactose derivative that has been chemically modified to include a mildly thiol-reactive chloromethyl group. Once inside a cell, the DetectaGene™ substrate undergoes two reactions: 1) its two galactose moieties are cleaved by intracellular β-galactosidase and 2) either simultaneously or sequentially, its chloromethyl moiety reacts with glutathione and possibly other intracellular thiols to form a membrane-impermeant, peptide-fluorescent dye adduct.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Substrate: CMFDG
Assay Type: β-Gal (lacZ) Assay
Product Size: 1 kit
Target Enzyme: Beta-Galactosidase
Substrate Type: Beta-Gal Substrate
Detection Method: Fluorescent
Substrate Properties: Chemical Substrate
Excitation⁄Emission (nm): 492⁄517
Shipping Condition: Room Temperature

Contents & storage

Store in freezer (-5 to -30°C) and protect from light.

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