DiA; 4-Di-16-ASP (4-(4-(Dihexadecylamino)styryl)-N-Methylpyridinium Iodide)
DiA; 4-Di-16-ASP (4-(4-(Dihexadecylamino)styryl)-<i>N</i>-Methylpyridinium Iodide)
Citas y referencias (37)
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DiA; 4-Di-16-ASP (4-(4-(Dihexadecylamino)styryl)-N-Methylpyridinium Iodide)

La DiA de sonda de aminosteril lipofílica se inserta en las membranas mediante sus dos colas de alquilo y suMás información
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Número de catálogoCantidad
D3883
también denominado D-3883
25 mg
Número de catálogo D3883
también denominado D-3883
Precio (MXN)
-
Cantidad:
25 mg
La DiA de sonda de aminosteril lipofílica se inserta en las membranas mediante sus dos colas de alquilo y su fluoróforo orientado paralelamente a la cadena de acilo de fosfolípido. Cuando esta sonda de dialquil aminosteril se une a las membranas, muestra una fuerte mejora de la fluorescencia; su fluorescencia en el agua es mínima.
Para uso exclusivo en investigación. No apto para uso en procedimientos diagnósticos.
Especificaciones
ColorAmarillo
Método de detecciónFluorescente
Para utilizar con (equipo)Microscopio de fluorescencia
Cantidad25 mg
Condiciones de envíoTemperatura ambiente
Tipo de productoDiA 4-Di-16-ASP
SubCellular LocalizationMembranas celulares y lípidos, Lipids
Unit SizeEach
Contenido y almacenamiento
Almacenar a temperatura ambiente y proteger de la luz.

Preguntas frecuentes

I want to label the plasma membrane of my cells, but there are several dyes to choose from. Which one should I use?

For live-cell imaging, the CellVue and CellMask Plasma Membrane Stains are the most uniform and the slowest to be endocytosed. However, they are not the best choice if you wish to fix and permeabilize your cells, such as for antibody labeling. Wheat germ agglutinin (WGA) conjugates are also able to label live cells, or can label already formaldehyde-fixed cells. They can survive subsequent permeabilization with detergents, such as Triton X-100. If cells are already permeabilized, WGA will label internal structures as well. Thus, only an antibody against a plasma membrane protein can be used if cells are already permeabilized. Lipophilic cyanine dyes, such as DiI, will label all cell membranes in live cells, not just plasma membranes, if left on live cells for extended periods. Following page will help you choose (http://www.thermofisher.com/us/en/home/life-science/cell-analysis/cell-structure/plasma-membrane.html).

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Citations & References (37)

Citations & References
Abstract
Carbocyanine postmortem neuronal tracing. Influence of different parameters on tracing distance and combination with immunocytochemistry.
Authors:Lukas JR, Aigner M, Denk M, Heinzl H, Burian M, Mayr R
Journal:J Histochem Cytochem
PubMed ID:9671441
'Carbocyanines (DiI, DiA, DiO) are able to travel along membranes by diffusion and therefore have been used as postmortem neuronal tracers in aldehyde-fixed tissues. Surprisingly, detailed data on the influence of different parameters on tracing distances are still missing. This study was carried out to optimize tracing procedures and to ... More
Interhemispheric asymmetries of the modular structure in human temporal cortex.
Authors:Galuske RA, Schlote W, Bratzke H, Singer W
Journal:Science
PubMed ID:10988077
'Language-relevant processing of auditory signals is lateralized and involves the posterior part of Brodmann area 22. We found that the functional lateralization in this area was accompanied by interhemispheric differences in the organization of the intrinsic microcircuitry. Neuronal tract tracing revealed a modular network of long-range intrinsic connections linking regularly ... More
The location of fluorescence probes with charged groups in model membranes.
Authors:Kachel K, Asuncion-Punzalan E, London E
Journal:Biochim Biophys Acta
PubMed ID:9814853
'The location of commonly used charged fluorescent membrane probes in membranes was determined in order to: (1) investigate the relationship between the structure of hydrophobic molecules and their depth within membranes; and (2) aid interpretation of experiments in which these fluorescent probes are used to examine membrane structure. Membrane depth ... More
Cortical radial glial cells in human fetuses: depth-correlated transformation into astrocytes.
Authors:deAzevedo LC, Fallet C, Moura-Neto V, Daumas-Duport C, Hedin-Pereira C, Lent R
Journal:J Neurobiol
PubMed ID:12717699
'In the human brain, the transformation of radial glial cells (RGC) into astrocytes has been studied only rarely. In this work, we were interested in studying the morphologic aspects underlying this transformation during the fetal/perinatal period, particularly emphasizing the region-specific glial fiber anatomy in the medial cortex. We have used ... More
Vagal efferent and afferent innervation of the rat esophagus as demonstrated by anterograde DiI and DiA tracing: focus on myenteric ganglia.
Authors:Neuhuber WL, Kressel M, Stark A, Berthoud HR
Journal:J Auton Nerv Syst
PubMed ID:9686909
'Anterograde tracing with the carbocyanine tracer DiI and the aminostyrol derivative DiA was used to selectively label fibers from the nucleus ambiguus, dorsal motor nucleus and nodose ganglion, respectively, terminating in the rat esophagus, and to compare them with the innervation of the gastric fundus in the same animals. Ambiguus ... More
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