Invitrogen™

DDAO Phosphate (9H-(1,3-Dichloro-9,9-Dimethylacridin-2-One-7-yl) Phosphate, Diammonium Salt)

Catalog number:  D6487

Related applications:

Labeling Chemistry

| Pharma & Biopharma

| Protein Assays and Analysis

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Description

The phosphatase substrate DDAO phosphate [9H-(1,3-dichloro-9,9-dimethylacridin-2-one-7-yl) phosphate, diammonium salt] yields a hydrolysis product that can be excited with the 633 nm laser (excitation/emission maxima ~645/660). Although the substrate itself is fluorescent (excitation/emission maxima ~460/610 nm), the difference between the substrate's excitation maximum and that of its hydrolysis product is over 200 nm, allowing the two species to be easily distinguished.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Excitation/Emission: 646⁄659
Form: Powder
Label or Dye: DDAO
Shipping Condition: Room Temperature
Substrate: DDAO Phosphate
Substrate Properties: Chemical Substrate
Substrate Type: Phosphatase Substrate
Target Enzyme: Phosphatases
Detection Method: Fluorescent
For Use With (Equipment): Fluorometer, Microplate Reader

Contents & storage

Store in freezer (-5 to -30°C) and protect from light.