Thermo Scientific™

FitBag™ Half Fraser Broth

 Related applications:
Food & Beverage
Industrial Microbiology
Save time in preparing selective enrichment for the isolation of Listeria spp. from food and environmental samples using Thermo Scientific™ FitBag™ Half Fraser Broth. The irradiated medium is supplied in lightweight, transparent, plastic bags—just add water and the medium is ready for use. The bag’s segmented structure allows pre-filling so that you can reconstitute in two minutes when needed. Available in 2.7L, 4.5L or 9L sizes to suit your workflow, medium can be dispensed through the outlet tube as required.

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Description

Shorten your workflow with FitBag Half Fraser Broth. FitBag Enrichment Media is quick, easy-to-prepare, and highly adaptable to laboratory workflows and automated equipment, such as dilutors. No further supplementation is required.

Cost-effective: no need for weighing of media or sterilization, reducing the burden of media preparation, which frees up resources and equipment. Unprepared FitBag Enrichment Media can be kept for up to 24 months.

Flexible: segmented structure allows advanced preparation by filling one segment with deionized water from which FitBag Enrichment Media can be reconstituted within two minutes. Pre-sterilized water may also be added under aseptic conditions.

Easy-to-use:
fill and dispense with minimum training.

Energy saving: gamma irradiated, eliminating the need for costly thermal processing.

Safe: eliminate handling of glassware, weighing of culture media, and the need to add supplementary reagents. Empty bags can be disposed of with normal laboratory waste.

Quality guaranteed: compliant with ISO 11133 and ISO 7218. Quality certificates are available for every batch, reducing the QC testing needed.

Space-saving:
conveniently packed in flat boxes, FitBag Enrichment Media takes up relatively little space compared to prepared media and can be made up as required.

FitBag Enrichment Media is supplied without filters. We recommend the autoclavable Thermo Scientific™ Dry-Bags™ Reusable Filter (DB0200A). For added convenience, it is recommended that bags are filled using the Thermo Scientific Peristaltic Pump (DB2000A or DB3000A) and dispensed using the Thermo Scientific Automated Diluter (DB4150A).

Half Fraser Broth is a modification of the USDA-FSIS UVM (University of Vermont Medium) secondary enrichment broth and is based on the formula described by Fraser and Sperber1. Blackening of the medium is presumptive evidence of the presence of Listeria spp. All Half Fraser Broth enrichment cultures should be subcultured to plating medium.
1. Fraser J.A. and Sperber W.H. (1988) J. Food Protect. 51, No.10, 762-765.
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