Invitrogen™

Epidermal Growth Factor, Biotinylated, complexed to Alexa Fluor™ 488 Streptavidin (Alexa Fluor™ 488 EGF complex)

Catalog number: E13345
Invitrogen™

Epidermal Growth Factor, Biotinylated, complexed to Alexa Fluor™ 488 Streptavidin (Alexa Fluor™ 488 EGF complex)

Catalog number: E13345
Catalog Number
E13345
Unit Size
100 µg
Price (USD)
Price: 532.00
Your Price:
Availability
-
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
E13345100 µg
Price: 532.00
Your Price:
-
Product Overview
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We offer several conjugates of epidermal growth factor that are useful for the detection of EGF receptor in cells. Fluorescently labeled EGF has enabled scientists to investigate receptor-membrane interactions, study receptor distribution, calculate rate constants for the interaction of EGF with its receptor, and more. The conjugates incorporating fluorescein (FITC), Oregon Green™ 514, and tetramethylrhodamine were constructed with the dye directly attached to EGF. The conjugates of EGF with Alexa Fluor™ dyes and Texas Red™ dye are complexes of the dye–streptavidin molecule and biotinylated EGF. The biotinylated conjugates utilize biotin-XX, which contains a long spacer arm designed to enhance the probe’s affinity for the EGF receptor.

Epidermal Growth Factor Conjugate Specifications:
• EGF molecule: 53 amino acids, MW=6,045 Da
• Label (Ex/Em): Biotin-XX complexed with Alexa Fluor™ 488 streptavidin (∼495/519 nm)
• Number of fluorophore molecules on each EGF molecule: ∼2–3
• Fluorescence is typically monitored using a flow cytometer, fluorescence microscope, or fluorimeter


Find More Receptor Binding and Phagocytosis Probes
We offer a number of fluorescently labeled probes for studying receptor-mediated endocytosis, membrane markers for endocytosis and exocytosis, and methods for detecting internalized fluorescent ligands. Review Probes for Following Receptor Binding and Phagocytosis—Section 16.1 in the Molecular Probes™ Handbook for more information on these products.

For Research Use. Not for human or animal therapeutic or diagnostic use.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Cell Type
Mammalian Cell
Detection Method
Fluorescent
Product Type
Growth Factor
Dye Type
Alexa Fluor Dyes
Form
Solid
Product Line
Alexa Fluor™
Shipping Condition
Wet Ice
Protein Family
EGF

Contents & Storage

Store in freezer (-5 to -30°C) and protect from light.