A facile method for immunofluorescence microscopy of highly autofluorescent human retinal sections using nanoparticles with large Stokes shifts.
AuthorsPetty HR, Elner VM, Kawaji T, Clark A, Thompson D, Yang DL,
JournalJ Neurosci Methods
PubMed ID20619292
'The human retina is rich in autofluorescent species, such as lipofuscin and melanin. Consequently, it is difficult to localize antigens in the human retina using immunofluorescence microscopy. To address this issue, we have developed a methodology to tag retinal antigens using quantum dot nanoparticles that absorb in the ultraviolet and ... More
Prevention of nonspecific binding of avidin.
AuthorsDuhamel RC, Whitehead JS
JournalMethods Enzymol
PubMed ID2388571
Suppression of endogenous avidin-binding activity in tissues and its relevance to biotin-avidin detection systems.
AuthorsWood GS, Warnke R
JournalJ Histochem Cytochem
PubMed ID7028859
As biotin-avidin systems continue to be developed for applications involving single cells, cell suspensions, and especially tissue sections, the need arises for a method of blocking endogenous avidin-binding activity. One such method is described and its proposed mechanism is discussed. Utilizing this method, endogenous avidin-binding activity was detected and suppressed ... More
Simultaneous inhibition of endogenous avidin-binding activity and peroxidase applicable for the avidin-biotin system using monoclonal antibodies.
AuthorsMatsumoto Y
JournalHistochemistry
PubMed ID2415495
The use of the avidin-biotin technique in immunoperoxidase staining provides a simple and highly sensitive method for detecting the localization of antigens defined by monoclonal antibodies. However, endogenous biotin, which is widely distributed in tissues, often causes non-specific staining by binding to avidin [endogenous avidin-binding activity (EABA)]. Endogenous peroxidase activity ... More