EnzChek® Peptidase/Protease Assay Kit

Catalog number: E33758

Molecular Probes™  Related applications: Cellular Imaging | Protein Assays and Analysis

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Description

The EnzChek® Peptidase/Protease Assay Kit enables simple and accurate quantification of a wide range of protease activities within 60 minutes.

See our complete line of Fluorescence Microplate assays.

• Simple mix-incubate-read format
• Excitation and emission wavelengths (502/528 nm) well suited for fluorescein (FITC) filter settings
• Easily adapted to a variety of proteases and reaction conditions

The EnzChek® peptidase/protease substrate included in the kit has been labeled with a fluorophore and a quencher moiety separated by a peptide. Upon cleavage by protease(s), the fluorophore separates from the quencher and, upon excitation, the fluorophore emits fluorescence signal. The magnitude of the resultant signal is proportional to the degree of substrate cleavage, resulting in an ability to quantify the amount of enzyme activity. The assay is performed in a simple mix–incubate–read format, can be completed in 60 minutes or less, and is easily adapted to accommodate diverse pH requirements.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Technique: Fluorescence Intensity
Assay Type: Protease Assay
Product Size: 1 kit
Target Enzyme: Peptidases, Proteases
Substrate Type: Protease Substrate, Peptidase Substrate
Substrate Properties: Peptide-Based Substrate
Shipping Condition: Room Temperature

Contents & storage

Store in freezer (-5 to -30°C) and protect from light.

Documents

Manuals & protocols