Invitrogen™

EnzChek™ Pyrophosphate Assay Kit

Catalog number: E6645
Invitrogen™

EnzChek™ Pyrophosphate Assay Kit

Catalog number: E6645
Catalog Number
E6645
Unit Size
100 assays
Price (USD)
477.00
Your Price:
Availability
***
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
E6645100 assays
477.00
Your Price:
***
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The EnzChek™ Pyrophosphate Assay Kit provides a rapid and highly sensitive enzymatic assay for detecting free pyrophosphate in solution. In this assay the formation of a chromophoric product is detected spectrophotometrically.

See our complete line of Fluorescence Microplate assays.

• Sensitivity limit of 1 µM pyrophosphate (∼0.2 µg/mL)
• Acommodates a pH range of 6.5 to 8.5

The EnzChek™ Pyrophosphate Detection Kit provides a fast, convenient, and inexpensive spectrophotometric method for measuring the inorganic pyrophosphate produced by a number of biochemical reactions, such as DNA and RNA polymerizations, cyclic AMP formation by the enzyme adenylate cyclase, and the enzymatic activation of fatty acids to form their coenzyme A esters.

The EnzChek™ Pyrophosphate Assay Kit is a modification of our EnzChek™ Phosphate Assay Kit (Cat. No. E6646). In the presence of inorganic phosphate, the substrate 2-amino-6-mercapto-7-methylpurine ribonucleoside (MESG) is converted by the purine nucleoside phosphorylase (PNP) enzyme to ribose 1-phosphate and 2-amino-6-merc apto-7-methylpurine. The enzymatic conversion of MESG results in a shift in absorbance maximum from 330 nm to 360 nm. The EnzChek™ Pyrophosphate Assay Kit includes the enzyme inorganic pyrophosphatase, which catalyzes conversion of pyrophosphate into two equivalents of phosphate. The phosphate is then consumed by the MESG/PNP reaction and detected by an increase in absorbance at 360 nm. Additional sensitivity is gained by the amplification of one molecule of pyrophosphate into two molecules of phosphate.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Color
Ultraviolet
Detection Method
Colorimetric
For Use With (Application)
Pyrophosphate Assay
For Use With (Equipment)
Spectrophotometer
Product Type
Pyrophosphate Assay
Dye Type
MESG
Format
Tube(s)
Product Line
EnzChek™
Shipping Condition
Room Temperature

Contents & Storage

Includes inorganic pyrophosphatase, 2-Amino-6-mercapto-7-methylpurine riboside (MESG), purine nucleoside phosphorylase (PNP), concentrated reaction buffer, Na2P2O7 standard, and detailed protocols for detecting and quantitating pyrophosphate.

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