Thermo Scientific™

BL21(DE3) Competent Cells

Catalog number: EC0114
Thermo Scientific™

BL21(DE3) Competent Cells

Catalog number: EC0114
Catalog Number
EC0114
Unit Size
10 x 100 µL
Price (USD)
132.00
Your Price:
Availability
***
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
EC011410 x 100 µL
132.00
Your Price:
***
Product Overview
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Additional Information
Recommendations
Recommendations
Thermo Scientific BL21(DE3) Competent Cells are suitable for the expression of non-toxic heterologous genes. The strain contains the lambda DE3 prophage that carries the gene for T7 RNA polymerase under control of a lacUV5 promoter, allowing expression of the T7 RNA polymerase to be induced with IPTG. BL21(DE3) is an E. coli B strain and does not contain the lon protease. It is also deficient in the outer membrane protease OmpT. The lack of these two key proteases reduces degradation of heterologous proteins expressed in the cells.

• High transformation efficiency: >1x107 cfu/µg pUC19 DNA
• Specifically constructed for high-level expression of recombinant proteins
• Genetic markers that minimize RNA and protein degradation
• Convenient two reactions per vial packaging
• S.O.C. Outgrowth medium included

Applications
BL21(DE3) Competent Cells are suitable for high-level T7 expression of recombinant proteins.

Genotype
F– ompT hsdSB (rB–, mB–) gal dcm (DE3)

Note: Not suitable for maintenance of expression plasmids. Use DH10B or DH5α competent cells that do not carry the gene for T7 RNA polymerase for plasmid DNA propagation (Cat. Nos. EC0112, EC0113).
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Product Type
Competent Cell
Efficiency
High Efficiency (>1x10^7 cfu/µg pUC19 DNA)
Cell Type
Chemically Competent
Cloning Methylated DNA
Yes
Bacterial or Yeast Strain
BL21(DE3)
Blue/White Screening
No
Shipping Condition
Dry Ice
Species
E. coli
Promoter
LacUV5
Format
Tube
Product Line
Thermo Scientific™
Quantity
10 x 100 μL

Contents & Storage

• 10 x BL21(DE3) Competent Cells (100 µL)
• pUC19 DNA (50 µL) (10 pg/µL)
• S.O.C. Medium (5 mL)

Store at -80°C.

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