Novex™ 12% Zymogram (Casein) Protein Gels, 1.0 mm, 12-well

Catalog number: EC64052BOX

Invitrogen™  Related applications: Protein Gel Electrophoresis

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Novex® Zymogram Gels are excellent tools for detecting and characterizing proteases that utilize casein or gelatin as a substrate. Proteases are run on a gel containing one of these substrates under denaturing conditions and visualized as clear bands against a dark background following a simple renaturing, developing, and staining protocol. Zymogram gels are commonly used to detect matrix metalloproteinases.

Formulation: All Novex® Zymogram Gels are Tris-Glycine gels with a substrate (gelatin or casein) incorporated into the gel. They are made with high-purity, strictly quality-controlled reagents: Tris base, HCl, acrylamide, bisacrylamide, TEMED, APS, highly purified water and casein or gelatin as the substrate. All have a 4% stacking gel.

Zymogram Gel Types: The 10% Zymogram (Gelatin) Gel is a 10% Tris-Glycine gel with 0.1% gelatin as the substrate. The 12% Novex® Zymogram (Casein) Gel is a 12% Tris-Glycine gel with 0.05% casein as the substrate. The 4-16% Novex® Zymogram (Blue Casein) Gel is a 4-16% Novex® Tris-Glycine gel with 0.1% casein as the substrate and a proprietary dye incorporated throughout the gel.

Sensitivity Level:
10% Zymogram Gelatin Gel: 5 x 10-6 units of collagenase
12% Zymogram Casein Gel: 7 x 10-4 units of trypsin
4-16% Zymogram Casein Gel: 1.5 x 10-3 units of trypsin

Recommended Buffers: Use Novex® Tris-Glycine SDS Sample Buffer and Novex® Tris-Glycine SDS Running Buffer with these gels. Novex® Zymogram Renaturing and Developing Buffers are also recommended.
For Research Use Only. Not for use in diagnostic procedures.


For Use With (Equipment): Mini Gel Tank, XCell SureLock™ Mini
Gel Percentage: 12 %
Gel Size: Mini (8 cm x 8 cm)
Gel Type: Zymogram Gel
Mode of Separation: Molecular Weight
Product Line: Novex™
Product Size: 10 gels (1 box)
Quantity: 10 gels
Sample Loading Volume: 20 µL
Separation Range: 20 to 120 kDa (Tris-Glycine Buffer)
Substrate: Casein
Substrate Type: Protease Substrate
System: Novex®
Thickness: 1.0 mm
Well Format: 1D Well
Wells: 12 wells
Shipping Condition: Wet Ice

Contents & storage

One box contains 10 gels. Store in refrigerator (2–8°C). Do not freeze. Shelf life is 8 weeks.


Manuals & protocols

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