VEGF Human ELISA Kit

Catalog number: EH2VEGF

Invitrogen™  Related applications: Protein Assays and Analysis

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Description

Assay VEGF-A (human vascular endothelial growth factor) levels in human serum, plasma (heparin, sodium citrate) and culture supernatant samples with our Thermo Scientific Human VEGF ELISA Kit.

Features of the Human VEGF ELISA Kit:

Target: natural and recombinant human VEGF-A (vascular endothelial growth factor); see Product Details for additional information
Format: colorimetric ELISA (TMB substrate) in a clear 96-well strip plate
Measurement: absorbance at 450nm minus absorbance at 550nm
Assay Range: 31.3 to 2000 pg/mL
Sensitivity: < 5 pg/mL
Precision: intra-assay CV < 10%; inter-assay CV < 10%
Sample Types: cell culture supernatant, serum, plasma (EDTA, sodium citrate and heparin)
Total Assay Time: 4 hours
Sample Size: 50 µL
Specificity: no significant cross-reaction with human VEGF ELISA: human IL-1α, IL-1β, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-11, IL-12, IL-12p40, IL-13, IL-15, IL-16, IL-17, PLGF-1, PLGF-2, RANTES, TGFβ, TNFβ, VEGF-B, VEGF-C or VEGF-D; rat VEGF; mouse VEGF120 or VEGF164.

This human VEGF-A ELISA kit uses pre-titered, matched pairs of coating and detection antibodies to achieve sensitive and accurate measurement of vascular endothelial growth factor (VEGF-A) in samples at 50 µL per well. Also included are calibrated recombinant protein standard, streptavidin-HRP, TMB substrate solution and stop solution. The complete kit contains a pre-coated 96-well strip plate and lot-optimized reagents. All components undergo quality control testing to minimize lot-to-lot variability.

Includes:
Complete Kit contains pre-coated antibody-plates, detection antibodies, buffers, diluents, standards, and substrates.

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For Research Use Only. Not for use in diagnostic procedures.

Specifications

Protein Family: Growth Factors & Receptors
Assay Range: See product documentation
Assay Sensitivity: See product documentation
Detection Method: Colorimetric
For Use With (Equipment): Microplate Reader
Former Identifiers: Pierce
Gene Aliases: MVCD1, VEGF, VPF
Gene ID: 7422
Gene Name: vascular endothelial growth factor A
Gene Symbol: VEGFA
Immunoassay Kit Format: Coated ELISA Kit
Label or Dye: HRP (Horseradish Peroxidase)
Product Size: 96 tests
Protein Aliases: VEGF-A, Vascular permeability factor, VPF
Protein Name: vascular endothelial growth factor A
Target (Kit Named): VEGF
Target Species (Named): Human
Target Species (Validated): Human
UniProt ID: P15692

Contents & storage

Pre-coated plate(s), standard or calibrator, detector antibody, HRP conjugate, diluents, wash buffer, substrate(s), and stop solution. Store kit at 2-8°C. See product manual for detailed contents and storage conditions for maximum stability.

Documents

Manuals & protocols