GM-CSF ELISA Kit, Human

Catalog number: EHGMCSF

Thermo Scientific™  Related applications: Protein Assays and Analysis

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Description

The Thermo Scientific Human GM-CSF ELISA Kit contains sufficient reagents and buffers to assay GM-CSF (granulocyte macrophage colony-stimulating factor) levels in human serum, plasma, urine and culture supernatants. This Thermo Scientific Human GM-CSF ELISA Kit is sufficient to assay 96 microwells.

Features of the Thermo Scientific Human GM-CSF ELISA Kit:

Target: natural and recombinant human granulocyte-macrophage colony-stimulating factor (GM-CSF
Format: colorimetric ELISA (TMB substrate) in clear 96-well strip plate
Measurement: absorbance at 450nm minus absorbance at 550nm
Assay Range: 15.4 to 600 pg/mL
Sensitivity: < 2 pg/mL
Reproducibility: intra-assay CV ≤ 10%; inter-assay CV ≤ 10%
Sample Types: cell culture supernatant, urine, serum, plasma (EDTA, sodium citrate and heparin)
Total Assay Time: 4 hours
Sample Size: 50 µL
Specificity: no significant cross-reaction with human IL-1α, IL-1β, IL-2, IL-3, IL-6, IL-7, IL-8, IFNγ, TNFα, or mouse GM-CSF.

Includes:
Complete kits contain pre-coated antibody-plates, detection antibodies, buffers, diluents, standards, and substrates.

Specifications

Product Size: Each
Description: Orion Star A214 pH/ISE benchtop meter kit with fluoride electrode and solutions, ROSS Ultra glass-body pH electrode, ATC probe, stirrer probe and stand
Model: Orion Star A214 pH/ISE/mV/RmV/ORP/Temperature Benchtop Meter
Item Description: Orion Star A214 pH/ISE benchtop meter kit with fluoride electrode and solutions, ROSS Ultra glass-body pH electrode, ATC probe, stirrer probe and stand
Includes: Star A214 pH/ISE Meter with Electrode Stand; Universal Power Adapter; 8102BNUWP ROSS Ultra Glass Refillable pH Electrode; 927007MD Stainless Steel ATC Probe; 9609BNWP Fluoride Electrode; 096019 Stirrer Probe; 040906 Fluoride 1ppm with TISAB II Standard; 040907 2ppm with TISAB II Standard; 040908 Fluoride 10ppm with TISAB II Standard; 940909 TISAB II Solution; Electrode Arm with redesigned holder;  Universal Power Adapter
Range (pH): -2.000 to 20.000pH
Accuracy (pH): ±0.002pH
Resolution (pH): 0.1, 0.01, 0.001pH
Range (mV): ±2000.0mV
Range (Relative mV): ±2000.0mV
Range (ORP): ±2000.0mV
Resolution (mV): 0.1mV
Accuracy (mV): ±0.2 mV or ±0.05% of reading whichever is greater
Range (ISE): 0.0001 to 19900 with selectable units of ppm, Molar, mg/L, %, ppb or none
Resolution (ISE): 0.0001 minimum, 1 to 3 significant digits (user selectable)
Accuracy (ISE): ±0.2 mV or ±0.05% of reading whichever is greater
Advanced ISE Functions: Segmented (point-to-point) slope, non-linear selectable auto-blank, low concentration range stability
Calibration: pH with calibration editing option, ion concentration (ISE) with calibration editing option, relative mV (RmV), ORP and temperature
Calibration Points (pH): 1 to 5
Calibration Points (ISE): 1 to 5
Calibration Points: 1 point relative mV (RmV) mode, 1 point ORP mode
Isopotential Point: 7.000pH
Range (Temperature): -5.0° to 105.0° C, 22.0° to 221.0° F
Resolution (Temperature): 0.1°C, 0.1°F
Accuracy (Temperature): ±0.1°C
Temperature Selection: Manual or automatic with ATC temperature probe
Temperature Probe Calibration: 1 point temperature offset calibration
Channels: 1
Backlight Option: Yes, selectable
Display Type: Graphic LCD with backlight
Keypad: Comprehensive with menu-specific function keys and dual purpose scroll/shortcut keys
Measurement Modes: pH, mV, relative mV (RmV), ORP or ion concentration (ISE) with temperature
Stability: Selectable fast, medium, slow or Smart Stability setting; Selectable off or Automatic Smart averaging setting
Data Management Details: Transfer single, range or all data points to printer or computer
Log Function Type: Automatic data logging with Auto-Read and Timed measure modes; manual data logging with Continuous measure mode
Data Points: 2000 with date and time stamp
Alarm Output: High/low limit alarm, calibration due alarm
Memory: Non-volatile memory preserves data log, calibration log and meter settings
Methods: 10 per channel with password protection
Inputs: BNC (pH, ORP or ion selective electrode), pin-tip (reference electrode), 8 pin MiniDIN (ATC temperature probe), stirrer input (stirrer probe)
Outputs: USB, RS232
Languages: English, Spanish, French, Italian, German and Chinese; Portuguese and Korean available with software update
AC Adapter: Universal 50-60 Hz, 100-240 VAC power adapter (Cat. No. 1010003)
Power Supply: Universal AC adapter (included) or 4 AA batteries (sold separately)
Battery Life: 800 hours
Battery Type: Four AA (sold separately)
IP Rating: IP-54
Temperature (English) Operating: 41° to 113°F (ambient)
Temperature (Metric) Operating: 5° to 45° C (ambient)
Relative Humidity Range: 5 to 85 %, non-condensing
Dimensions (L x W x H): 24cm x 18cm x 11cm (9.5 in. x 7.1 in. x 4.3 in.)
Weight (English): 2 lb.
Weight (Metric): 0.9kg
Certifications/Compliance: CE, TUV 3-1, FCC Class A
Warranty: 3 year meter warranty

Contents & storage

Pre-coated plate(s), standard or calibrator, detector antibody, HRP conjugate, diluents, wash buffer, substrate(s), and stop solution. Store kit at 2-8°C. See product manual for detailed contents and storage conditions for maximum stability.

Documents

Manuals & protocols