GM-CSF Human ELISA Kit

Catalog number: EHGMCSF5

Invitrogen™  Related applications: Protein Assays and Analysis

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Description

The Thermo Scientific Human GM-CSF ELISA Kit contains sufficient reagents and buffers to assay GM-CSF (granulocyte macrophage colony-stimulating factor) levels in human serum, plasma, urine and culture supernatants. This Thermo Scientific Human GM-CSF ELISA Kit is sufficient to assay five 96 microwell plates.

Features of the Thermo Scientific Human GM-CSF ELISA Kit:

Target: natural and recombinant human granulocyte-macrophage colony-stimulating factor (GM-CSF
Format: colorimetric ELISA (TMB substrate) in clear 96-well strip plate
Measurement: absorbance at 450nm minus absorbance at 550nm
Assay Range: 15.4 to 600 pg/mL
Sensitivity: < 2 pg/mL
Reproducibility: intra-assay CV ≤ 10%; inter-assay CV ≤ 10%
Sample Types: cell culture supernatant, urine, serum, plasma (EDTA, sodium citrate and heparin)
Total Assay Time: 4 hours
Sample Size: 50 µL
Specificity: no significant cross-reaction with human IL-1α, IL-1β, IL-2, IL-3, IL-6, IL-7, IL-8, IFNγ, TNFα, or mouse GM-CSF.

Includes:
Complete kits contain pre-coated antibody-plates, detection antibodies, buffers, diluents, standards, and substrates.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Assay Sensitivity: See product documentation
Detection Method: Colorimetric
For Use With (Equipment): Microplate Reader
Former Identifiers: Pierce
Gene Aliases: GMCSF
Gene ID: 1437
Gene Name: colony stimulating factor 2
Gene Symbol: CSF2
Immunoassay Kit Format: Coated ELISA Kit
Label or Dye: HRP (Horseradish Peroxidase)
Product Size: 5 x 96 tests
Protein Aliases: GM-CSF, Colony-stimulating factor, CSF, Molgramostin, Sargramostim
Protein Name: Granulocyte-macrophage colony-stimulating factor
Target (Kit Named): GM-CSF
Target Species (Named): Human
Target Species (Validated): Human
UniProt ID: P04141

Contents & storage

Pre-coated plate(s), standard or calibrator, detector antibody, HRP conjugate, diluents, wash buffer, substrate(s), and stop solution. Store kit at 2-8°C. See product manual for detailed contents and storage conditions for maximum stability.

Documents

Manuals & protocols