IL-17A ELISA Kit, Human

Catalog number: EHIL17A

Thermo Scientific™  Related applications: Protein Assays and Analysis

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Description

The Human IL17A ELISA research-use-only kit is to be used for the quantitative determination of IL17A in samples (see sample types and species reactivity indicated) using 96-well plates and a microplate reader. The assay will recognize both natural and recombinant forms of this target.

Performance characteristics

• Sensitivity: 5 pg/mL
• Standard curve range: 31.25 pg/mL - 2000 pg/mL
• Sample types: serum, plasma, cell culture supernatant
• Specificity: This ELISA is specific for the measurement of natural and recombinant human IL17A. No significant crossreaction with human IL17B, IL17D, IL17E, IL17F, IL2, IL4, IL5, IL6, IL8, IL10, IL12, IL13, IL16 or mouse IL17A, IL1A, IL1B or GMCSF was found.
• Sample volume: 50 µL
• Total assay incubation time: 3.5 hours

Principle of the method
The Thermo Scientific™ Pierce™ Human IL17A ELISA is a solid-phase, sandwich-type enzyme-linked immunosorbent assay (ELISA) designed to measure the amount of the target between a 'sandwich' or two layers of antibodies. A target-specific antibody has been pre-coated onto the wells of the microtiter plate provided. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, binding to the target on a different epitope from the capture antibody. A conjugated enzyme has been incorporated into the assay. After incubation and washing steps to rid the microplate of unbound substances, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.

Background information
IL17A, also known as Interleukin 17, is a proinflammatory cytokine secreted by activated memory CD4+ T-cells. IL17A is noted for involvement in chronic inflammatory diseases, including psoriasis, multiple sclerosis and rheumatoid arthritis.

Related links:
Learn more about ELISA kits.
Learn more about other immunoassays.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Gene Aliases: IL-17A, IL-17, IL17, CTLA8
Accession Number: NP_002181.1
Gene ID: 3605.0
Species: Human
Gene Symbol: IL17A
Product Size: 96 assays
Protein Family: Cytokines & Receptors
Detection Method: Colorimetric
Research Category: Immunology, Apoptosis

Contents & storage

Pre-coated plate(s), standard or calibrator, detector antibody, HRP conjugate, diluents, wash buffer, substrate(s), and stop solution. Store kit at 2-8°C. See product manual for detailed contents and storage conditions for maximum stability.

Documents

Manuals & protocols

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