IFN gamma Mouse ELISA Kit

Catalog number: EM10012

Invitrogen™  Related applications: Protein Assays and Analysis

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Description

Assay IFN-γ (mouse interferon gamma) levels in mouse serum, plasma (EDTA, heparin, sodium citrate), and culture supernatant samples with our Thermo Scientific Mouse IFN gamma ELISA Kit.

Features of the Thermo Scientific Mouse IFN gamma ELISA Kit:

Target: natural and recombinant mouse interferon gamma (IFNγ)
Format: colorimetric ELISA (TMB substrate) in clear 96-well strip plate
Measurement: absorbance at 450nm minus absorbance at 550nm
Assay Range: 37 to 3000 pg/mL
Sensitivity: < 10 pg/mL
Precision: intra-assay CV < 10%; inter-assay CV < 10%
Sample Types: cell culture supernatant, serum, plasma (EDTA, sodium citrate and heparin)
Total Assay Time: 4 hours
Sample Size: 50 µL
Specificity: no significant cross-reaction with with mouse IL-1α, IL-1β, IL-3, IL-4, IL-6, IL-7, IL-10, GM-CSF, TNFα or human IFNα or IFNγ

This mouse IFN-gamma ELISA kit uses pre-titered, matched pairs of coating and detection antibodies to achieve sensitive and accurate measurement of interferon gamma (IFNγ) in samples at 50 to 100 µL per well. Also included are calibrated recombinant protein standard, streptavidin-HRP, TMB substrate solution and stop solution. The complete kit contains precoated 96-well strip plate(s) and lot-optimized reagents. All components undergo quality control testing to minimize lot-to-lot variability.

Note: Assay range and other values listed above are for the pre-coated plate kit; see product documentation for values specific to the reagent kit and antibody-pair kit.

Includes:
Complete Kit contains pre-coated antibody-plates, detection antibodies, buffers, diluents, standards, and substrates.

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For Research Use Only. Not for use in diagnostic procedures.

Specifications

Assay Sensitivity: See product documentation
Detection Method: Colorimetric
For Use With (Equipment): Microplate Reader
Former Identifiers: Pierce
Gene Aliases: IFN-g, Ifg
Gene ID: 15978
Gene Name: interferon gamma
Gene Symbol: IFNG
Immunoassay Kit Format: Coated ELISA Kit
Label or Dye: HRP (Horseradish Peroxidase)
Product Size: 2 x 96 tests
Protein Aliases: IFN-gamma
Protein Name: Interferon gamma
Target (Kit Named): IFN gamma
Target Species (Named): Mouse
Target Species (Validated): Mouse
UniProt ID: P01580

Contents & storage

Pre-coated plate(s), standard or calibrator, detector antibody, HRP conjugate, diluents, wash buffer, substrate(s), and stop solution. Store kit at 2-8°C. See product manual for detailed contents and storage conditions for maximum stability.

Documents

Manuals & protocols