IL-12 p70 (Total) Mouse ELISA Kit

Catalog number: EMIL12TOT2

Invitrogen™  Related applications: Protein Assays and Analysis

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Description

Assay IL-12 p70 (active mouse interleukin-12, p70) levels in mouse serum and culture supernatant samples with our Thermo Scientific Mouse IL-12 (p70) ELISA Kit.

Features of the Mouse IL-12 (p70) ELISA Kit:

Target: biologically active (p70) natural and recombinant mouse interleukin-12 (IL-12, p70)
Format: colorimetric ELISA (TMB substrate) in clear 96-well strip plate
Measurement: absorbance at 450nm minus absorbance at 550nm
Assay Range: 12.3 to 1000 pg/mL
Sensitivity: < 5 pg/mL
Precision: intra-assay CV < 10%; inter-assay CV < 10%
Sample Types: cell culture supernatant, serum
Total Assay Time: 3 hours
Sample Size: 50 µL
Specificity: no significant cross-reaction with mouse IL-1α, IL-1β, IL-3, IL-4, IL-5, IL-6, IL-7, TNFα, IFNγ, GM-CSF, or human IL-12 (p70). Recombinant mouse IL-12 (p40) and human IL-12 (p40) do not cross-react or interfere with this assay.

This mouse IL-12 ELISA kit uses pre-titered, matched pairs of coating and detection antibodies to achieve sensitive and accurate measurement of biologically active interleukin-12 (IL-12 p70) in samples at 50 µL per well. Also included are calibrated recombinant protein standard, streptavidin-HRP, TMB substrate solution and stop solution. The complete kit contains pre-coated 96-well strip plates and lot-optimized reagents. All components undergo quality control testing to minimize lot-to-lot variability.

Includes:
Complete kit contains pre-coated antibody-plates, detection antibodies, buffers, diluents, standards, and substrates.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Assay Sensitivity: See product documentation
Detection Method: Colorimetric
For Use With (Equipment): Microplate Reader
Former Identifiers: Pierce
Gene Aliases: IL-12p35, Il-12a, Ll12a, p35, Il-12b, Il-12p40, Il12p40, p40
Gene ID: 16159, 16160
Gene Name: interleukin 12A, interleukin 12b
Gene Symbol: IL12A, IL12B
Immunoassay Kit Format: Coated ELISA Kit
Label or Dye: HRP (Horseradish Peroxidase)
Product Size: 2 x 96 tests
Protein Aliases: IL-12A, Cytotoxic lymphocyte maturation factor 35 kDa subunit, CLMF p35, IL-12 subunit p35, IL-12B, Cytotoxic lymphocyte maturation factor 40 kDa subunit, CLMF p40, IL-12 subunit p40
Protein Name: Interleukin-12 subunit alpha, Interleukin-12 subunit beta
Target (Kit Named): IL-12 p70
Target Species (Named): Mouse
Target Species (Validated): Mouse
Target Sub-Specificity: Total
UniProt ID: P43431, P43432

Contents & storage

Pre-coated plate(s), standard or calibrator, detector antibody, HRP conjugate, diluents, wash buffer, substrate(s), and stop solution. Store kit at 2-8°C. See product manual for detailed contents and storage conditions for maximum stability.

Documents

Manuals & protocols