IL-4 ELISA Kit, Mouse

Catalog number: EMIL4

Thermo Scientific™  Related applications: Protein Assays and Analysis

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Description

Use the Thermo Scientific® Mouse IL-4 ELISA Kit to assay IL-4 (mouse interleukin-4) levels in mouse serum and cell culture supernatant samples. This is an alternate product for Cat. No. KMC0041.

This kit uses pre-titered, matched pairs of coating and detection antibodies to achieve sensitive and accurate measurement of IL-4 levels in samples. The Thermo Scientific® Mouse IL-4 ELISA Kit also includes calibrated recombinant protein standard, TMB substrate solution, and stop solution. This complete kit contains one pre-coated 96-well strip plate and lot-optimized reagents. All components undergo quality control testing to minimize lot-to-lot variability.

Highlights:
• Target: natural and recombinant mouse interleukin-4 (IL-4)
• Assay range: 15 to 375 pg/mL
• Sensitivity: <5 pg/mL
• Sample types: cell culture supernatant, serum
• Total assay time: 3.5 hours
• Sample size: 50 μL
• Specificity: no significant cross-reaction with with mouse IL-2, IL-3, IL-5, IL-6, IL-7, IFNγ, GM-CSF, TNFα; rat IL-4; rabbit IL-4; or human IL-4
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Gene Aliases: BSF-1, IL-4
Accession Number: NM_021283.2, NP_067258.1 
Gene ID: 16189
Species: Mouse
Product Size: 96 assays
Protein Family: Cytokines & Receptors
Detection Method: Colorimetric
Research Category: Immunology

Contents & storage

Pre-coated plate(s), standard or calibrator, detector antibody, HRP conjugate, diluents, wash buffer, substrate(s), and stop solution. Store kit at 2-8°C. See product manual for detailed contents and storage conditions for maximum stability.

Documents

Manuals & protocols