IL-4 Mouse ELISA Kit

Catalog number: EMIL45

Invitrogen™  Related applications: Protein Assays and Analysis

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Description

Assay IL-4 (mouse interleukin-4) levels in mouse serum and culture supernatant samples with our Thermo Scientific Mouse IL-4 ELISA Kit.

Features of the Mouse IL-4 ELISA Kit:

Target: natural and recombinant mouse interleukin-4 (IL-4)
Format: colorimetric ELISA (TMB substrate) in clear 96-well strip plates
Measurement: absorbance at 450nm minus absorbance at 550nm
Assay Range: 15 to 375 pg/mL
Sensitivity: < 5 pg/mL
Precision: intra-assay CV < 10%; inter-assay CV < 10%
Sample Types: cell culture supernatant, serum
Total Assay Time: 3.5 hours
Sample Size: 50 µL
Specificity: no significant cross-reaction with with mouse IL-2, IL-3, IL-5, IL-6, IL-7, IFNγ, GM-CSF, TNFα rat IL-4; rabbit IL-4; or human IL-4.

This mouse IL-4 ELISA kit uses pre-titered, matched pairs of coating and detection antibodies to achieve sensitive and accurate measurement of interleukin-4 (IL-4) in samples at 50 to 100 µL per well. Also included are calibrated recombinant protein standard, TMB substrate solution and stop solution. The complete kit contains pre-coated 96-well strip plates and lot-optimized reagents. All components undergo quality control testing to minimize lot-to-lot variability.

Includes:
Complete Kit contains pre-coated antibody-plates, detection antibodies, buffers, diluents, standards, and substrates.

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For Research Use Only. Not for use in diagnostic procedures.

Specifications

Assay Sensitivity: See product documentation
Detection Method: Colorimetric
For Use With (Equipment): Microplate Reader
Former Identifiers: Pierce
Gene Aliases: BSF-1, IL-4
Gene ID: 16189
Gene Name: interleukin 4
Gene Symbol: IL4
Immunoassay Kit Format: Coated ELISA Kit
Label or Dye: HRP (Horseradish Peroxidase)
Product Size: 5 x 96 tests
Protein Name: Interleukin-4
Target (Kit Named): IL-4
Target Species (Named): Mouse
Target Species (Validated): Mouse
UniProt ID: P07750

Contents & storage

Pre-coated plate(s), standard or calibrator, detector antibody, HRP conjugate, diluents, wash buffer, substrate(s), and stop solution. Store kit at 2-8°C. See product manual for detailed contents and storage conditions for maximum stability.

Documents

Manuals & protocols