p38 MAPK (Phospho) [pT180/pY182] Multispecies ELISA Kit

Catalog number: EMSP38P

Invitrogen™  Related applications: Protein Assays and Analysis

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Description

Assay phosphorylated p38 ([pThr180/Tyr182] p38 MAP kinase, pp38) levels in cell lysates of human and mouse origin with our Thermo Scientific Phospho p38 ELISA Kit.

Features of the Phospho p38 ELISA Kit:

Target: phosphorylated p38 ([pThr180/Tyr182] p38 MAP kinase, phospho-p38); also called SAP kinase 2 (SAPK2)
Format: colorimetric ELISA (TMB substrate) in clear 96-well strip plate
Measurement: absorbance at 450nm minus absorbance at 550nm
Assay Range: 156 to 5000 pg/mL
Sensitivity: < 52.1 pg/mL
Precision: dependent upon sample type and level (see instructions)
Sample Types: cell lysates of human and mouse origin
Total Assay Time: 3 hours
Sample Size: 100 µL
Specificity: no significant cross-reaction with non-phosphorylated p38, phospho-JNK, non-phosphorylated JNK, AKT, non-phosphoylated ERK, phospho-ERK.

This phospho-p38 ELISA kit is an immunoassay for the quantitative determination of phosphorylated p38 ([pThr180/Tyr182] p38 MAP kinase, pp38) in cell lysates. A recombinant phospho-p38 standard is provided with the kit. The assay uses a monoclonal antibody to p38 precoated in a microplate to bind the phospho-p38 in the standards or sample. After a short incubation, the excess sample or standard is washed out of the plate and a phospho p38 detection antibody is added. This antibody binds to the phospho-p38 captured on the plate. After a short incubation, the excess antibody is washed out and secondary conjugate is added, which binds to the phospho-p38 detection antibody. Excess conjugate is washed out and TMB Substrate is added. After a short incubation, the enzyme reaction is stopped and the absorbance is read at 450nm. The measured optical density is directly proportional to the concentration of phospho-p38 in either standards or samples.

Includes:
Complete kit contains pre-coated antibody-plate, detection antibodies, buffers, standard, substrate, and stop solution.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Protein Family: Other Proteins
Assay Range: See product documentation
Assay Sensitivity: See product documentation
Detection Method: Colorimetric
For Use With (Equipment): Microplate Reader
Former Identifiers: Pierce
Gene Aliases: CSBP, CSBP1, CSBP2, CSPB1, EXIP, Mxi2, PRKM14, PRKM15, RK, SAPK2A, p38, p38ALPHA
Gene ID: 1432
Gene Name: mitogen-activated protein kinase 14
Gene Symbol: MAPK14
Immunoassay Kit Format: Coated ELISA Kit
Label or Dye: HRP (Horseradish Peroxidase)
Product Size: 96 tests
Protein Name: Mitogen-activated protein kinase 14
Target (Kit Named): p38 MAPK
Target Site-Specificity: [pT180/pY182]
Target Species (Named): Multispecies
Target Species (Validated): Human, Mouse
Target Sub-Specificity: Phospho
UniProt ID: Q16539

Contents & storage

Pre-coated plate(s), standard or calibrator, detector antibody, HRP conjugate, diluents, wash buffer, substrate(s), and stop solution. Store kit at 2-8°C. See product manual for detailed contents and storage conditions for maximum stability.

Documents

Manuals & protocols