Thermo Scientific™

Lambda Exonuclease (10 U/µL)

Catalog number:  EN0561

 Related applications:

PCR Cloning

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Thermo Scientific Lambda Exonuclease is a highly processive 5'→3' exodeoxyribonuclease. It selectively digests the 5'-phosphorylated strand of double-stranded DNA. The enzyme exhibits low activity on single-stranded DNA and non-phosphorylated DNA, and has no activity at nicks and limited activity at gaps in DNA.


• Active in Thermo Scientific PCR buffers

• Generating single-stranded PCR products for use in: • DNA sequencing
• Analysis of DNA single-strand conformation polymorphism (SSCP)
• Rolling circle amplification

• Producing single-stranded DNA from double-stranded DNA fragments
• Cloning of PCR products


Use of this enzyme in certain applications may be covered by patents and may require a license.
For Research Use Only. Not for use in diagnostic procedures.


Enzyme: Exonuclease
Product Size: 1,000 units


Manuals & protocols