Informational:For PCR enzyme with ≤0.01 copy of bacterial gDNA per enzyme unit, check out Invitrogen Platinum Taq DNA Polymerase, DNA-free.
Thermo Scientific™

Taq DNA Polymerase, native, with BSA (5 U/µL)

Catalog number: EP0072
Thermo Scientific™

Taq DNA Polymerase, native, with BSA (5 U/µL)

Catalog number: EP0072
Catalog Number
EP0072
Unit Size
500 units
Price (USD)
Price: 243.00
Your Price:
Availability
***
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
EP0072500 units
Price: 243.00
Your Price:
***
Product Overview
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Additional Information
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Thermo Scientific Native Taq DNA Polymerase is a highly thermostable DNA polymerase of the thermophilic bacterium Thermus aquaticus. The enzyme catalyzes 5'→3' synthesis of DNA, has no detectable 3'→5' exonuclease (proofreading) activity and possesses low 5'→3' exonuclease activity. In addition, the polymerase exhibits deoxynucleotidyl transferase activity, which frequently results in the addition of extra adenines at the 3'-end of PCR products. Native Taq DNA Polymerase is preferred for amplification of bacterial DNA sequences homologous to those found in E. coli.

Taq DNA Polymerase (native, with BSA) is supplied with BSA as a stabilizing agent. This version of Taq DNA Polymerase is often the best choice when amplifying DNA samples of lower purity, e.g. genomic DNA from mouse tail.

Highlights

Thermostable—half life is more than 40 min at 95°C.
Generates PCR products with 3'-dA overhangs.
Supplied with two buffers—10X Taq Buffer with KCl and 10X Taq Buffer with (NH4)2SO4. The latter allows for PCR at wide range of magnesium concentrations and decreases unspecific priming.
Incorporates modified nucleotides (e.g., biotin-, digoxigenin-, fluorescently-labeled nucleotides).

Applications

• Routine PCR amplification of DNA fragments up to 5 kb
• DNA labeling (see Reference s 1-3)
• High throughput PCR

Note

• The error rate of Taq DNA Polymerase in PCR is 2.2 x 10-5 errors per nt per cycle. Accordingly, the accuracy of PCR is 4.5 x 104. Accuracy is an inverse of the error rate and shows an average number of correct nucleotides incorporated before an error occurs.
• The 10X Taq Buffer without Detergent is recommended for microarray experiments.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Concentration
5 U/µL
Polymerase
Taq DNA Polymerase
Compatible Buffer
10X Taq Buffer
Sensitive to Heat Inactivation
No
Optimal Reaction Temperature
72°C
Quantity
300 U
Product Type
DNA Polymerase
Research Category
PCR
Product Line
Thermo Scientific™
Inhibitors
Low-tolerance

Contents & Storage

Contains:
• 100 µL Taq DNA Polymerase, native (5 U/µL)
• 2 x 1.25 mL 10X Taq Buffer with KCl
• 2 x 1.25 mL 10X Taq Buffer with (NH4)2SO4
• 2 x 1.25 mL 25 mM MgCl2
• 0.25 mL 20 mg/mL BSA

Store at -20°C.

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