Thermo Scientific Bacteriophage T3 RNA polymerase is a DNA-dependent RNA polymerase with strict specificity for its respective double-stranded promoters. It catalyzes the 5'→3' synthesis of RNA on either single-stranded DNA or double-stranded DNA downstream from it promoter and is able to incorporate modified nucleotide.
• Synthesis of unlabeled and labeled RNA that can be used: • For hybridization, in vitro RNA translation • As aRNA, siRNA, substrate in RNase protection assays, template for genomic DNA sequencing • In studies of RNA secondary structure and RNA-protein interactions, RNA splicing
Consensus promoter sequence: T3: AATTAACCCTCACTAAAGGGAGA The position in bold (+1) indicates the first nucleotide incorporated into RNA during transcription. Only bases at this position through +3 are critical for transcription, and they must be a G and a purine base, respectively.
For Research Use Only. Not for use in diagnostic procedures.