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Thermo Scientific™
Taq DNA Polymerase, recombinant (5 U/μL)
Thermo Scientific Taq DNA Polymerase is a highly thermostable DNA polymerase from the thermophilic bacterium Thermus aquaticus.
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Catalog Number
Quantity
EP0405
5 x 500 Units
EP0401
100 Units
EP0402
500 Units
EP0406
10 x 500 Units
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Catalog number EP0405
Price (USD)/ 5 x 500 units
531.65
Online exclusive
616.00
Save 84.35 (14%)
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Quantity:
5 x 500 Units
Request bulk or custom format
Price (USD)/ 5 x 500 units
531.65
Online exclusive
616.00
Save 84.35 (14%)
Add to cart
Taq DNA Polymerase, recombinant (5 U/μL)
Catalog numberEP0405
Price (USD)/ 5 x 500 units
531.65
Online exclusive
616.00
Save 84.35 (14%)
-
Add to cart
Thermo Scientific Taq DNA Polymerase is a highly thermostable DNA polymerase from the thermophilic bacterium Thermus aquaticus. The enzyme catalyzes 5' to 3' synthesis of DNA, has no detectable 3' to 5' exonuclease (proofreading) activity, and possesses low 5' to 3' exonuclease activity. In addition, Taq DNA Polymerase exhibits deoxynucleotidyl transferase activity, which frequently results in the addition of extra adenines at the 3'-end of PCR products. Recombinant Taq DNA Polymerase is the ideal tool for standard PCR of templates 5 kb or shorter.
Features
Thermostable—half life is more than 40 min at 95°C
Generates PCR products with 3'-dA overhangs
Supplied with two buffers: 10X Taq Buffer with KCl and 10X Taq Buffer with (NH4)2SO4
Routine PCR amplification of DNA fragments up to 5 kb
High throughput PCR
DNA labeling
Notes
The error rate of Taq DNA Polymerase in PCR is 2.2 x 10-5 errors per nt per cycle. Accordingly, the accuracy of PCR is 4.5 x 104. Accuracy is an inverse of the error rate and shows an average number of correct nucleotides incorporated before an error occurs.
10X Taq Buffer without Detergent is recommended for microarray experiments.
10X Taq Buffer with (NH4)2SO4 allows for PCR at wide range of magnesium concentrations and decreases unspecific priming.
For Research Use Only. Not for use in diagnostic procedures.
In certain countries use of this product is covered by patents. Purchase of product in these countries includes non-transferable, limited license for using only this amount of product for the purchaser's own internal research.
Specifications
GC-Rich PCR PerformanceLow
PolymeraseTaq DNA Polymerase
Reaction SpeedStandard
Product TypeTaq DNA Polymerase (Recombinant)
Quantity5 x 500 Units
Shipping ConditionDry Ice
For Use With (Application)Standard PCR
Concentration5 U/μL
Fidelity (vs. Taq)1X
Hot StartNo
Overhang3'-A
Reaction FormatSeparate Components
Unit Size5 x 500 units
Contents & Storage
• Taq DNA Polymerase (5 U/μL), 5 x 100 μL • 10X Taq Buffer with KCl, 10 x 1.25 mL • 10X Taq Buffer with (NH4)2SO4, 10 x 1.25 mL • 25 mM MgCl2, 10 x 1.25 mL