DreamTaq Green DNA Polymerase (5 U/µL)

Catalog number: EP0714

Thermo Scientific™  Related applications: PCR

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Thermo Scientific DreamTaq Green DNA Polymerase is a combination of DreamTaq DNA Polymerase and 10X DreamTaq Green Buffer. DreamTaq DNA Polymerase is an enhanced Taq polymerase optimized for high throughput PCR applications. It ensures higher sensitivity, longer PCR products and higher yields compared to conventional Taq DNA polymerase. Extensive optimization of reaction conditions is not required.

The 10X DreamTaq Green Buffer includes a density reagent and two tracking dyes for direct loading of PCR products on a gel. The colored buffer does not interfere with PCR performance and is compatible with downstream applications such as DNA sequencing, ligation and restriction digestion. For applications requiring PCR product analysis by absorbance or fluorescence excitation, we recommend using the colorless 10X DreamTaq Buffer (#B65) or purifying the PCR product before analysis. The 10X DreamTaq Green Buffer is optimized for robust performance in PCR and includes MgCl2 at a concentration of 20 mM.


• Direct loading of PCR product on gel
• High yields and high sensitivity of PCR
• Amplification of long targets up to 6 kb from genomic DNA and up to 20 kb from viral DNA
• Robust amplification with minimal optimization
• Incorporates modified nucleotides, but does not incorporate dUTP


• Routine PCR amplification of DNA fragments up to 6 kb
• Genotyping
• High throughput PCR
For Research Use Only. Not for use in diagnostic procedures.


Polymerase: DreamTaq DNA Polymerase
Hot Start: No
Fidelity (vs. Taq): 1 X
GC-Rich PCR Performance: Low
Reaction Format: Standalone
Product Overhang: 3'-A
Reaction Speed: Standard
Product Size: 20 x 500 units

Contents & storage

• 20 x 100 µL DreamTaq DNA Polymerase (5 U/µL)
• 40 x 1.25 mL 10X DreamTaq Green Buffer (includes 20 mM MgCl2)

Store at -20°C.


Manuals & protocols

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