Maxima Reverse Transcriptase (200 U/μL)
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Thermo Scientific™
Maxima Reverse Transcriptase (200 U/μL)
Thermo Scientific Maxima Reverse Transcriptase (RT) was developed through in vitro evolution of M-MuLV RT. The enzyme possesses an RNA-dependentRead more
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Catalog NumberQuantity
EP07412,000 Units
EP074210,000 Units
EP07434 x 10,000 Units
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Catalog number EP0741
Price (USD)/ 2,000 units
73.65
Online exclusive
82.50 
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Quantity:
2,000 Units
Request bulk or custom format
Price (USD)/ 2,000 units
73.65
Online exclusive
82.50 
Save 8.85 (11%)
Add to cart
Maxima Reverse Transcriptase (200 U/μL)
Catalog numberEP0741
Price (USD)/ 2,000 units
73.65
Online exclusive
82.50 
Save 8.85 (11%)
-
Add to cart

Thermo Scientific Maxima Reverse Transcriptase (RT) was developed through in vitro evolution of M-MuLV RT. The enzyme possesses an RNA-dependent and DNA-dependent polymerase activity as well as RNase H activity. The engineered enzyme features dramatically improved thermostability and robustness, and increased synthesis rates compared to wild type M-MuLV RT.

Maxima Reverse Transcriptase is capable of reproducible cDNA synthesis from a wide range of input total RNA amounts (from 1 pg to 5 μg) at elevated temperatures (50–65°C), which makes this enzyme an ideal tool for two step RT-qPCR (see supporting data).

Due to its high thermostability, Maxima enzyme maintains full activity during the entire reverse transcription reaction, generates the highest yields of cDNA, and is able to synthesize even very long RNA transcripts up to 20 kb. The reaction temperature can be increased up to 65°C for efficient transcription of RNA regions with a high secondary structure or to improve specificity using gene specific primers. Due to its increased synthesis rate, the reverse transcriptase reaction can be completed in 15 to 30 minutes

Features of Maxima Reverse Transcriptase include:
• High yields of full-length cDNA up to 20 kb
• Active up to 65°C
• Thermostable—90% active after incubation at 50°C for 60 minutes
• High sensitivity allows reproducible cDNA synthesis from a wide range of total RNA quantities (1 pg to 5 μg)
• Efficient formula for complete cDNA synthesis in 15 to 30 minutes
• Incorporates modified nucleotides

Applications
• Two step RT-PCR
• Two step RT-qPCR
• First strand cDNA synthesis
• Construction of full length cDNA libraries
• DNA labeling
• Primer extension

Also available
Maxima Reverse Transcriptase
Maxima First Strand cDNA Synthesis Kit for RT-qPCR
Maxima First Strand cDNA Synthesis Kit for RT-qPCR, with dsDNase

For Research Use Only. Not for use in diagnostic procedures.
Specifications
FormatStand-alone Enzyme
GC-Rich PCR PerformanceHigh
Reaction Speed30 min.
TechniqueReverse Transcription
Optimal Reaction Temperature50°C to 55°C
Quantity2,000 Units
Reverse TranscriptaseMaxima
Ribonuclease H ActivityYes
Shipping ConditionDry Ice
Concentration200 U/μL
Final Product TypeFirst-Strand cDNA
Reaction FormatSeparate components
Reagent TypeReverse Transcription
Size (Final Product)Up to 20 kb
Starting MaterialRNA
Unit Size2,000 units
Contents & Storage

• Maxima Reverse Transcriptase, 1 x 2,000 units (200 U/μL)
• 5X RT Buffer

Store at –20°C.