TNF alpha ELISA Kit, Porcine

Catalog number: EP2TNFA

Thermo Scientific™  Related applications: Protein Assays and Analysis

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Description

Assay TNFa (porcine tumor necrosis factor alpha) levels in pig serum, plasma (EDTA, heparin, sodium citrate) and culture supernatant samples with our Thermo Scientific Porcine TNF alpha ELISA Kits.

These porcine TNF alpha ELISA kits use pre-titered, matched pairs of coating and detection antibodies to achieve sensitive and accurate measurement of tumor necrosis factor alpha (TNF-alpha, TNFα) in samples at 50 to 100 µL per well. Also included are calibrated recombinant protein standard, streptavidin-HRP, TMB substrate solution and stop solution. The complete kits contain precoated 96-well strip plate(s) and lot-optimized reagents. The economically-priced reagent kit provides validated reagents only, requiring users to coat their own plates. All components undergo quality control testing to minimize lot-to-lot variability.

Features:

Target: natural and recombinant porcine TNFα (porcine tumor necrosis factor alpha)
Format: colorimetric ELISA (TMB substrate) in clear 96-well strip plate
Measurement: absorbance at 450nm minus absorbance at 550nm
Assay Range: 31.3 to 2000 pg/mL
Sensitivity: < 5 pg/mL
Precision: dependent upon sample type and level (see instructions)
Sample Types: cell culture supernatant, serum, plasma (EDTA, sodium citrate and heparin)
Total Assay Time: 4 hours
Sample Size: 50 µL
Specificity: no significant cross-reaction with human IL-1α, IL-1β, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-11, IL-12p70, IL-12p40, IL-13, IL-15, IL-16, IL-17, IL-18, Eotaxin, GCSF, GMCSF, GROα, GROβ, IFNγ, IFNα, MCP-1, MCP-2, MCP-3, MCP-4, VEGF, MIP-1α, MIP-1β, TGFβ, TNFβ or RANTES; mouse TNFα rat TNFα bovine TNFα. This ELISA uses antibodies raised against human TNFα. This ELISA reacts with porcine and human TNFα at 100%.

Note: Assay range and other values listed above are for the pre-coated plate kit; see product documentation for values specific to the reagent kit.

Includes:
Complete Kits contain pre-coated antibody-plates, detection antibodies, buffers, diluents, standards, and substrates. The Reagent Kit (previously called a "Screening Set") contains matched antibodies, standard and detection reagents but no coated plates.

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For Research Use Only. Not for use in diagnostic procedures.

Specifications

Species: Swine⁄Porcine
Product Size: 96 assays
Protein Family: Cytokines & Receptors
Detection Method: Colorimetric
Research Category: Immunology

Contents & storage

Pre-coated plate(s), standard or calibrator, detector antibody, HRP conjugate, diluents, wash buffer, substrate(s), and stop solution. Store kit at 2-8°C. See product manual for detailed contents and storage conditions for maximum stability.

Documents

Manuals & protocols