TNF alpha ELISA Kit, Porcine

Catalog number: EP2TNFA2

Thermo Scientific™  Related applications: Protein Assays and Analysis

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Assay TNFa (porcine tumor necrosis factor alpha) levels in pig serum, plasma (EDTA, heparin, sodium citrate) and culture supernatant samples with our Thermo Scientific Porcine TNF alpha ELISA Kit.

Features of the Porcine TNF alpha ELISA Kit:

Target: natural and recombinant porcine TNFα (porcine tumor necrosis factor alpha)
Format: colorimetric ELISA (TMB substrate) in clear 96-well strip plates
Measurement: absorbance at 450nm minus absorbance at 550nm
Assay Range: 31.3 to 2000 pg/mL
Sensitivity: < 5 pg/mL
Precision: dependent upon sample type and level (see instructions)
Sample Types: cell culture supernatant, serum, plasma (EDTA, sodium citrate and heparin)
Total Assay Time: 4 hours
Sample Size: 50 µL
Specificity: no significant cross-reaction with human IL-1α, IL-1β, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-11, IL-12p70, IL-12p40, IL-13, IL-15, IL-16, IL-17, IL-18, Eotaxin, GCSF, GMCSF, GROα, GROβ, IFNγ, IFNα, MCP-1, MCP-2, MCP-3, MCP-4, VEGF, MIP-1α, MIP-1β, TGFβ, TNFβ or RANTES; mouse TNFα rat TNFα bovine TNFα. This ELISA uses antibodies raised against human TNFα. This ELISA reacts with porcine and human TNFα at 100%.

This porcine TNF alpha ELISA kit uses pre-titered, matched pairs of coating and detection antibodies to achieve sensitive and accurate measurement of tumor necrosis factor alpha (TNF-alpha, TNFα) in samples at 50 to 100 µL per well. Also included are calibrated recombinant protein standard, streptavidin-HRP, TMB substrate solution and stop solution. The complete kit contains precoated 96-well strip plates and lot-optimized reagents.
For Research Use Only. Not for use in diagnostic procedures.


Species: Swine⁄Porcine
Product Size: 192 assays
Protein Family: Cytokines & Receptors
Detection Method: Colorimetric
Research Category: Immunology

Contents & storage

Pre-coated plate(s), standard or calibrator, detector antibody, HRP conjugate, diluents, wash buffer, substrate(s), and stop solution. Store kit at 2-8°C. See product manual for detailed contents and storage conditions for maximum stability.


Manuals & protocols