TNF alpha Porcine ELISA Kit

Catalog number: EP2TNFA2

Invitrogen™  Related applications: Protein Assays and Analysis

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Description

Assay TNFa (porcine tumor necrosis factor alpha) levels in pig serum, plasma (EDTA, heparin, sodium citrate) and culture supernatant samples with our Thermo Scientific Porcine TNF alpha ELISA Kit.

Features of the Porcine TNF alpha ELISA Kit:

Target: natural and recombinant porcine TNFα (porcine tumor necrosis factor alpha)
Format: colorimetric ELISA (TMB substrate) in clear 96-well strip plates
Measurement: absorbance at 450nm minus absorbance at 550nm
Assay Range: 31.3 to 2000 pg/mL
Sensitivity: < 5 pg/mL
Precision: dependent upon sample type and level (see instructions)
Sample Types: cell culture supernatant, serum, plasma (EDTA, sodium citrate and heparin)
Total Assay Time: 4 hours
Sample Size: 50 µL
Specificity: no significant cross-reaction with human IL-1α, IL-1β, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-11, IL-12p70, IL-12p40, IL-13, IL-15, IL-16, IL-17, IL-18, Eotaxin, GCSF, GMCSF, GROα, GROβ, IFNγ, IFNα, MCP-1, MCP-2, MCP-3, MCP-4, VEGF, MIP-1α, MIP-1β, TGFβ, TNFβ or RANTES; mouse TNFα rat TNFα bovine TNFα. This ELISA uses antibodies raised against human TNFα. This ELISA reacts with porcine and human TNFα at 100%.

This porcine TNF alpha ELISA kit uses pre-titered, matched pairs of coating and detection antibodies to achieve sensitive and accurate measurement of tumor necrosis factor alpha (TNF-alpha, TNFα) in samples at 50 to 100 µL per well. Also included are calibrated recombinant protein standard, streptavidin-HRP, TMB substrate solution and stop solution. The complete kit contains precoated 96-well strip plates and lot-optimized reagents.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Assay Sensitivity: See product documentation
Detection Method: Colorimetric
For Use With (Equipment): Microplate Reader
Former Identifiers: Pierce
Gene Aliases: TNFa
Gene ID: 397086
Gene Name: tumor necrosis factor
Gene Symbol: TNF
Immunoassay Kit Format: Coated ELISA Kit
Label or Dye: HRP (Horseradish Peroxidase)
Product Size: 2 x 96 tests
Protein Name: tumor necrosis factor
Target (Kit Named): TNF alpha
Target Species (Named): Porcine
Target Species (Validated): Porcine
UniProt ID: P23563

Contents & storage

Pre-coated plate(s), standard or calibrator, detector antibody, HRP conjugate, diluents, wash buffer, substrate(s), and stop solution. Store kit at 2-8°C. See product manual for detailed contents and storage conditions for maximum stability.

Documents

Manuals & protocols