IFN gamma ELISA Kit, Porcine

Catalog number: EPIFNG

Thermo Scientific™  Related applications: Protein Assays and Analysis

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Description

Assay IFN-γ (porcine interferon gamma) levels in pig serum, plasma (EDTA, heparin, sodium citrate), and culture supernatant samples with our Thermo Scientific Porcine IFN gamma ELISA Kit.

Features of the Porcine IFN gamma ELISA Kit:

Target: natural and recombinant porcine interferon gamma (IFNγ)
Format: colorimetric ELISA (TMB substrate) in clear 96-well strip plate
Measurement: absorbance at 450nm minus absorbance at 550nm
Assay Range: 8 to 500 pg/mL
Sensitivity: < 2 pg/mL
Sample Types: cell culture supernatant, serum, plasma (EDTA, sodium citrate and heparin)
Total Assay Time: 3 hours
Sample Size: 50 µL
Specificity: no significant cross-reaction with with porcine IL-1β, IL-6, IL-8, IL-12 (p40), IL-12 (p70), or human, mouse or rat IFNγ

This porcine IFN-gamma ELISA kit uses pre-titered, matched pairs of coating and detection antibodies to achieve sensitive and accurate measurement of interferon gamma (IFNγ) in samples at 50 µL per well. Also included are calibrated recombinant protein standard, streptavidin-HRP, TMB substrate solution and stop solution. The complete kit contains precoated 96-well strip plate(s) and lot-optimized reagents. All components undergo quality control testing to minimize lot-to-lot variability.

Includes:
Complete kit contains pre-coated antibody-plates, detection antibodies, buffers, diluents, standards, and substrates.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Species: Swine⁄Porcine
Product Size: 96 assays
Protein Family: Cytokines & Receptors
Detection Method: Colorimetric
Research Category: Immunology

Contents & storage

Pre-coated plate(s), standard or calibrator, detector antibody, HRP conjugate, diluents, wash buffer, substrate(s), and stop solution. Store kit at 2-8°C. See product manual for detailed contents and storage conditions for maximum stability.

Documents

Manuals & protocols