Invitrogen™

Cytokine/Chemokine/Growth Factor 11-Plex Canine ProcartaPlex™ Panel 1

Catalog number:  EPX11A-50511-901

Related applications:

Protein Assays and Analysis

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Description

The Canine Cytokine & Chemokine & Growth Factor 11-plex ProcartaPlex Panel is a preconfigured multiplex immunoassay kit that measures 11 protein targets using Luminex xMAP technology. ProcartaPlex preconfigured panels are supplied with all the necessary reagents to perform the assay and are validated to ensure reproducibility and consistency. These panels are designed for those who need greater validation for longitudinal studies.

ProcartaPlex immunoassays are based on the principles of a sandwich ELISA, using two highly specific antibodies binding to different epitopes of one protein to quantitate all protein targets simultaneously using a Luminex instrument. ProcartaPlex multiplex assays require as little as 25 µL of plasma or serum, or 50 µL of cell culture supernatant, and just four hours to obtain analyzed results.

Reproducible, reliable results—validated as a panel to the highest industry standard, including protein target combinability and cross-reactivity testing
More results per sample—measure multiple protein targets simultaneously in a single 25–50 µL sample
Well-established Luminex technology—the most referenced multiplexing platform for protein detection and quantitation

ProcartaPlex assays utilize Luminex xMAP (multianalyte profiling) technology for the simultaneous detection and quantitation of up to 80 protein targets in a single 25–50 µL sample—from plasma, serum, cell culture supernatants, and other bodily fluids.

The Luminex MagPlex superparamagnetic microsphere beads in the ProcartaPlex assay are internally dyed with precise proportions of red and infrared fluorophores to create 100 spectrally unique signatures that can be identified by the Luminex xMAP detec¬tion systems (Luminex 200, FLEXMAP 3D, and MAGPIX systems). Similar to a sandwich ELISA, the ProcartaPlex assay uses matched antibody pairs to identify the protein of interest. In a multiplexed assay, each spectrally unique bead is labeled with antibodies specific for a single target protein, and bound proteins are identified with biotinylated antibodies and streptavidin–R-phycoerythrin (RPE). The conjugation of protein-specific antibodies to a distinct bead allows for analysis of multiple targets in a single well.

The most significant difference between a ProcartaPlex assay and ELISA is that the capture antibody in the ProcartaPlex assay is conjugated to a magnetic bead and not adsorbed to the microplate well, so the ProcartaPlex assay reagents are free-floating in the solution. For detection, the Luminex 200 instrument, for example, contains two lasers, one to distinguish the spectral signature of each bead and the second to quantify the amount of RPE fluorescence, which is proportional to the amount of protein present in the sample. ProcartaPlex multiplex assays can profile more target proteins using significantly less sample in the same time that it takes to perform a traditional sandwich ELISA.

ProcartaPlex multiplex panels are available in multiple formats across six species (human, mouse, rat, nonhuman primate, porcine, and canine). Visit thermofisher.com/procartaplex for more information and available products.

Reactivity/Species
Canine

Suitable Sample Types
cell culture supernatant, serum, plasma

Sample Volume
Serum, Plasma: 25 µL; CCS: 50 µL

Reported Application
Multiplex Immunoassay

Target List
IFN gamma; IL-2; IL-6; IL-8; IL-10; IL-12p40; MCP-1; NGF beta; SCF; TNF alpha; VEGF-A

Bead Regions
See product documentation

Assay Range
As determined for Lot 1: IFN gamma: 6.37-26100 pg/mL; IL-2: 7.32-30000 pg/mL; IL-6: 12.13-49700 pg/mL; IL-8: 10.08-41300 pg/mL; IL-10: 8.59-35200 pg/mL; IL-12p40: 6.62-27100 pg/mL; MCP-1: 6.23-25500 pg/mL; NGF beta: 3.91-16000 pg/mL; SCF: 6.01-24600 pg/mL; TNF alpha: 4.25-17400 pg/mL; VEGF-A: 5.15-21100 pg/mL

Assay Sensitivity
See product documentation

For Research Use Only. Not for use in diagnostic procedures.

Specifications

Bead Region: See product documentation
Former Identifiers: eBioscience
Immunoassay Kit Format: Panel
Multiplex Panel Name: Cytokine/Chemokine/Growth Factor
Multiplex Panel Version: 1
Multiplex Size Plex No.: 11
Product Line: ProcartaPlex™
System Type: Luminex™
Target Kit Named: Cytokine/Chemokine/Growth Factor
Target List: IFN gamma; IL-2; IL-6; IL-8; IL-10; IL-12p40; MCP-1; NGF beta; SCF; TNF alpha; VEGF-A
Target Species Named: Canine
Target Species Primary: Canine
Target Species Validated: Canine
For Use With (Equipment): Luminex™ Instruments
Sample Volume: CCS: 50 µL, Serum, Plasma: 25 µL
Detection Method: Fluorescent
Interassay CV: Less than 10%
Intraassay CV: Less than 10%
Label or Dye: R-PE
Sample Type: Cell Culture Supernatants, Serum, Plasma

Contents & storage

2 vials Canine Cytokine/Chemokine/Growth Factor Standard Mix
1 vial Canine Cytokine/Chemokine/Growth Factor Bead Mix
1 vial Canine Cytokine/Chemokine/Growth Factor Detection Antibody Mix
1 bottle Reading Buffer
1 bottle Wash Buffer 10x
1 bottle Streptavidin-PE
1 bottle Universal Assay Buffer
1 bottle Detection Antibody Diluent
PCR 8-Tube Strip
Adhesive Film
Greiner Microplate black 96 well

Store at 2-8°C.