Invitrogen™

Immuno-Oncology Checkpoint 14-Plex Human ProcartaPlex™ Panel 2

Catalog number:  EPX140-15815-901

Related applications:

Protein Assays and Analysis

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Description

The Human Immuno-Oncology Checkpoint 14-Plex ProcartaPlex Panel 2 enables cancer research by analyzing 14 protein targets using Luminex xMAP technology. This panel allows simultaneous detection of soluble NK cell checkpoint molecules that regulate NK cell activation against tumor cells by the balance of inhibitory or activating signals. Analysis of these soluble biomarkers could help to shed light on the biology of the pathways. It complements Immuno-Oncology Checkpoint 14-Plex ProcartaPlex Panel 1 (Cat. No. EPX14A-15803-901) and Immuno-Oncology Checkpoint 10-Plex ProcartaPlex Panel 3 (Cat. No. EPX100-15820-901), providing 24 additional protein targets in the same research area.

ProcartaPlex preconfigured panels are extensively tested for analyte combinability, interference, and cross-reactivity to provide the highest level of validation and precision. All ProcartaPlex panels are supplied with the necessary reagents to perform the assay.

Target list [bead region]:
Activating: MICA [18], MICB [21], Perforin [53], ULBP-1 [73], ULBP-3 [77], ULBP-4 [78]
Inhibitory: Arginase-1 [51], CD73 (NT5E) [30], CD96 (Tactile) [35], E-Cadherin [44], Nectin-2 [29], PVR [56], Siglec-7 [12], Siglec-9 [13]

About ProcartaPlex assays for the Luminex platform
ProcartaPlex immunoassays are based on the principles of a sandwich ELISA, using two highly specific antibodies binding to different epitopes of one protein to quantitate all protein targets simultaneously using a Luminex instrument. ProcartaPlex multiplex assays require as little as 25 μL of plasma or serum, or 50 μL of cell culture supernatant, and just four hours to obtain analyzed results.

Features include:
• Reproducible, reliable results—validated as a panel to the highest industry standard, including protein target combinability and cross-reactivity testing
• More results per sample—measure multiple protein targets simultaneously in a single 25–50 μL sample
• Well-established Luminex technology highly referenced multiplexing platform for protein detection and quantitation

ProcartaPlex assays utilize Luminex xMAP (multianalyte profiling) technology for the simultaneous detection and quantitation of up to 65 protein targets in a single 25–50 μL sample—from plasma, serum, cell culture supernatants, and other bodily fluids.

The Luminex beads in the ProcartaPlex assay are internally dyed with precise proportions of red and infrared fluorophores to create spectrally unique signatures that can be identified by the Luminex xMAP detection systems (e.g., Luminex 200, FLEXMAP 3D, and MAGPIX). Similar to a sandwich ELISA, the ProcartaPlex assay uses matched antibody pairs to identify the protein of interest. In a multiplexed assay, each spectrally unique bead is labeled with antibodies specific for a single target protein, and bound proteins are identified with biotinylated antibodies and streptavidin–R-phycoerythrin (RPE). The conjugation of protein-specific antibodies to a distinct bead allows for analysis of multiple targets in a single well.

The most significant difference between a ProcartaPlex assay and ELISA is that the capture antibody in the ProcartaPlex assay is conjugated to a bead and not adsorbed to the microplate well, so the ProcartaPlex assay reagents are free-floating in the solution. For detection, the Luminex 200 instrument, for example, co

For Research Use Only. Not for use in diagnostic procedures.

Specifications

Assay Range: See Certificate of Analysis
Sample Volume: Serum, Plasma: 25 µL; CCS: 50 µL
Interassay CV: Less than 15%
Intraassay CV: Less than 15%
Quantity: 96 tests
Target Species: Human
Sample Type: Serum, Plasma, Cell Culture Supernatants
Multiplex Size Plex No.: 14
Shipping Condition: Wet Ice
Product Line: ProcartaPlex™
Immunoassay Kit Format: Multiplex Kit
For Use With (Equipment): Luminex™ Instruments

Contents & storage

• 2 vials Human Standard Mix O (lyophilized)
• 2 vials Perforin Standard (lyophilized)
• 1 vial Capture Bead Mix (1 X)
• 1 vial Biotinylated Detection Antibody Mix (50 X)
• 1 Bottle Reading Buffer (1 X)
• 1 Bottle Wash Buffer (10 x)
• 1 Bottle Streptavidin-PE (1 X)
• 1 Bottle Universal Assay Buffer (1 X)
• 1 Bottle Detection Antibody Diluent (1 X)
• 8-tube strip
• Adhesive film
• Flat-bottom 96-well plate, black

Store (at 2°C to 8°C.)