IL-1 beta ELISA Kit, Rat

Catalog number: ER2IL1B

Thermo Scientific™  Related applications: Protein Assays and Analysis

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Use the Thermo Scientific® Rat IL-1 beta ELISA Kit to assay IL-1β (rat interleukin-1 beta) levels in rat serum, plasma (with EDTA, heparin, sodium citrate), and cell culture supernatant samples. This is an alternate product for Cat. No. KRC0011.

This kit uses pre-titered, matched pairs of coating and detection antibodies to achieve sensitive and accurate measurement of IL-1β levels in samples. The Thermo Scientific® Rat IL-1 beta ELISA Kit also includes calibrated recombinant protein standard, streptavidin-HRP, TMB substrate solution, and stop solution. This complete kit contains one pre-coated 96-well strip plate and lot-optimized reagents. All components undergo quality control testing to minimize lot-to-lot variability.

• Target: natural and recombinant rat interleukin-1 beta (IL-1β)
• Assay range: 25.6 to 2500 pg/mL
• Sensitivity: <12 pg/mL
• Sample types: cell culture supernatant, serum, plasma (with EDTA, sodium citrate, heparin)
• Total assay time: 3.5 hours
• Sample size: 50 μL
• Specificity: no significant cross-reaction with rat IFNγ, IL-1α, IL-2, IL-4, IL-6, IL-10, MCP-1, MIP-1α, or RANTES, or human, mouse or pig IL-1β
For Research Use Only. Not for use in diagnostic procedures.


Gene Aliases: IL1B
Accession Number: NP_113700.2, NM_031512.2
Gene ID: 24494
Species: Rat
Product Size: 96 assays
Protein Family: Cytokines & Receptors
Detection Method: Colorimetric
Research Category: Immunology

Contents & storage

Pre-coated plate(s), standard or calibrator, detector antibody, HRP conjugate, diluents, wash buffer, substrate(s), and stop solution. Store kit at 2-8°C. See product manual for detailed contents and storage conditions for maximum stability.


Manuals & protocols