MCP-1 ELISA Kit, Rat

Catalog number: ERMCP12

Thermo Scientific™  Related applications: Protein Assays and Analysis

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Assay MCP-1 (rat monocyte chemoattractant protein-1) levels in rat serum, plasma (EDTA, heparin, sodium citrate) and culture supernatant samples with our Thermo Scientific Rat MCP-1 ELISA Kit.

Features of the Rat MCP-1 ELISA Kit:

Target: natural and recombinant rat MCP-1 (monocyte chemoattractant protein-1)
Format: colorimetric ELISA (TMB substrate) in clear 96-well strip plates
Measurement: absorbance at 450nm minus absorbance at 550nm
Assay Range: 38 to 1500 pg/mL
Sensitivity: < 5 pg/mL
Precision: intra-assay CV < 10%; inter-assay CV < 10%
Sample Types: cell culture supernatant, serum, plasma (EDTA, sodium citrate and heparin)
Total Assay Time: 3 hours
Sample Size: 50 µL
Specificity: no significant cross-reaction with rat IFNγ, IL-1α, IL-1β, IL-2, IL-4, IL-6, IL-10, MIP-1α, RANTES, mouse MCP-1, or human MCP-1.

The Rat MCP-1 ELISA Kit uses pre-titered, matched pairs of coating and detection antibodies to achieve sensitive and accurate measurement of monocyte chemoattractant protein-1 (MCP-1) in samples at 50 µL per well. Also included are calibrated recombinant protein standard, streptavidin-HRP, TMB substrate solution and stop solution. This complete kit contains pre-coated 96-well strip plates and lot-optimized reagents. All components undergo quality control testing to minimize lot-to-lot variability.

Complete kit contains pre-coated antibody-plates, detection antibodies, buffers, diluents, standards, and substrates.
For Research Use Only. Not for use in diagnostic procedures.


Species: Rat
Product Size: 192 assays
Protein Family: Cytokines & Receptors
Detection Method: Colorimetric
Research Category: Immunology

Contents & storage

Pre-coated plate(s), standard or calibrator, detector antibody, HRP conjugate, diluents, wash buffer, substrate(s), and stop solution. Store kit at 2-8°C. See product manual for detailed contents and storage conditions for maximum stability.


Manuals & protocols