IL-1 beta ELISA Reagent Kit, Human

Catalog number: ESS0008

Thermo Scientific™  Related applications: Protein Assays and Analysis

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The Thermo Scientific Human IL-1 beta ELISA Reagent Kit contains matched antibodies and sufficient reagents to assay IL-1β (human interleukin-1 beta) levels in human serum, plasma (heparin, sodium citrate), urine and culture supernatants. Each kit contains capture and detection antibodies, Streptavidin-HRP, TMB substrate and Stop solutions, and sufficient IL-1α standard to generate a standard curve with replicates

Features of the Thermo Scientific Human IL-1 beta ELISA Reagent Kit:

Target: natural and recombinant human interleukin-1 beta (IL-1β)
Format: colorimetric ELISA (TMB substrate) in clear 96-well strip plate
Measurement: absorbance at 450nm minus absorbance at 550nm
Assay Range: 10.2 to 400 pg/mL
Sensitivity: ≤ 1 pg/mL
Precision: intra-assay CV ≤ 10%; inter-assay CV ≤ 10%
Sample Types: cell culture supernatant, serum, plasma (sodium citrate and heparin)
Total Assay Time: 4 hours
Sample Size: 50 µL
Specificity: no significant cross-reaction with human IL-1α, IL-1RA, IL-2, IL-3, IL-4, IL-6, IL-7, IL-8, TNFα, IFNα, IFNγ or mouse IL-1β

Note: Assay range and other values listed above are for the pre-coated plate kit; see product documentation for values specific to the reagent kit and antibody-pair kit.

These human IL-1 beta ELISA kits use pre-titered, matched pairs of coating and detection antibodies to achieve sensitive and accurate measurement of interleukin-1 beta (IL-1β) in samples at 50 to 100 µL per well. Also included are calibrated recombinant protein standard, streptavidin-HRP, TMB substrate solution and stop solution. The complete kits contain pre-coated 96-well strip plate(s) and lot-optimized reagents. The economically-priced reagent kit provides validated reagents only, requiring users to coat their own plates. The antibody-pair kit consists of the matched IL-1β antibody pairs and IL-1β standard only. All components undergo quality control testing to minimize lot-to-lot variability.

Complete Kits contain pre-coated antibody-plates, detection antibodies, buffers, diluents, standards, and substrates. The Reagent Kit (previously called a "Screening Set") contains matched antibodies, standard and detection reagents but no coated plates. The Antibody Pair Kit (previously called a "MiniKit") contains only matched antibodies and standard (no plates or detection reagents).

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For Research Use Only. Not for use in diagnostic procedures.


Species: Human
Product Size: 480 assays
Protein Family: Cytokines & Receptors
Detection Method: Colorimetric
Research Category: Immunology

Contents & storage

Pre-coated plate(s), standard or calibrator, detector antibody, HRP conjugate, diluents, wash buffer, substrate(s), and stop solution. Store kit at 2-8°C. See product manual for detailed contents and storage conditions for maximum stability.


Manuals & protocols