IL-2 ELISA Reagent Kit, Human

Catalog number: ESS0010

Thermo Scientific™  Related applications: Protein Assays and Analysis

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The Thermo Scientific Human IL-2 ELISA Reagent Kit contains matched antibodies and sufficient reagents to assay human IL-2 levels in human serum, plasma (heparin, sodium citrate, EDTA) urine and culture supernatants. Each kit contains capture and detection antibodies, Streptavidin-HRP, TMB substrate and Stop solutions, and sufficient IL-2 standard to generate a standard curve with replicates

Features of the Thermo Scientific Human IL-2 ELISA Reagent Kit :

Target: natural and recombinant human interleukin-2 (IL-2)
Format: colorimetric ELISA (TMB substrate) in clear 96-well strip plate
Measurement: absorbance at 450nm minus absorbance at 550nm
Assay Range: 38.4 to 1500 pg/mL
Sensitivity: < 6 pg/mL
Precision: intra-assay CV < 10%; inter-assay CV < 10%
Sample Types: cell culture supernatant, serum, plasma (EDTA, sodium citrate and heparin)
Total Assay Time: 4 hours
Sample Size: 50 µL
Specificity: no significant cross-reaction with human IL-1α, IL-1β, IL-3, IL-4, IL-6, IL-7, IL-8, TNFα, TNFβ, GM-CSF, INFγ, or mouse IL-2.

Note: Assay range and other values listed above are for the pre-coated plate kit; see product documentation for values specific to the reagent kit and antibody-pair kit.

These human IL-2 ELISA kits use pre-titered, matched pairs of coating and detection antibodies to achieve sensitive and accurate measurement of interleukin-2 (IL-2) in samples at 50 to 100 µL per well. Also included are calibrated recombinant protein standard, streptavidin-HRP, TMB substrate solution and stop solution. The complete kits contain pre-coated 96-well strip plate(s) and lot-optimized reagents. The economically-priced reagent kit provides validated reagents only, requiring users to coat their own plates. The antibody-pair kit consists of the matched IL-2 antibody pairs and IL-2 standard only. All components undergo quality control testing to minimize lot-to-lot variability.

Complete Kits contain pre-coated antibody-plates, detection antibodies, buffers, diluents, standards, and substrates. The Reagent Kit (previously called a "Screening Set") contains matched antibodies, standard and detection reagents but no coated plates. The Antibody Pair Kit (previously called a "MiniKit") contains only matched antibodies and standard (no plates or detection reagents).

» Compare ELISA Kit formats »
For Research Use Only. Not for use in diagnostic procedures.


Species: Human
Product Size: 480 assays
Protein Family: Cytokines & Receptors
Detection Method: Colorimetric
Research Category: Immunology

Contents & storage

Standard, coating antibody, detection antibody, streptavidin-HRP, substrate, and stop solution. Store kit at 2-8°C. See product manual for detailed contents and storage conditions for maximum stability.


Manuals & protocols