Thermo Scientific™

Phire Hot Start II DNA Polymerase

Catalog number: F122S
Thermo Scientific™

Phire Hot Start II DNA Polymerase

Catalog number: F122S
Catalog Number
F122S
Unit Size
200 reactions
Price (USD)
Price: 190.00
Your Price:
Availability
-
Quantity
Catalog Number
Unit Size
1,000 reactions
Price (USD)
Price: 800.00
Your Price:
Availability
-
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
F122S200 reactions
Price: 190.00
Your Price:
-
F122L1,000 reactions
Price: 800.00
Your Price:
-
Product Overview
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Thermo Scientific Phire Hot Start II DNA Polymerase is an enhanced PCR enzyme for routine and high throughput PCR applications. It outperforms every Taq-based hot start polymerase on the market. Phire Hot Start II DNA Polymerase is significantly faster, extremely robust, and also capable of amplifying long DNA fragments with high yields. These features are achieved through advanced protein engineering of the polymerase. It incorporates a unique double-stranded DNA binding domain which allows short extension times (10 to 15 s/kb), improves yields, and increases fidelity 2-fold compared to Taq DNA polymerase.

The hot start modification of Phire Hot Start II DNA Polymerase is based on the Affibody inactivation technology. This technology increases the specificity of the PCR reaction with no additional time required for initial activation of the enzyme.

Highlights

Quick hot start—No reactivation step
Fast enzyme—Amplify four times faster than with hot start Taq
Robust—Minimal reaction optimization due to high inhibitor tolerance
High yields—Abundant products due to high efficiency
Longer PCR products—Amplify significantly longer DNA fragments than with any hot start Taq

Applications

• Hot Start PCR
• Routine PCR
• Non-high fidelity PCR
• Fast PCR
• High throughput PCR
• Genotyping

Note:The optimal annealing temperature for Phire DNA Polymerases may differ significantly from that of Taq-based polymerases. For optimal results start by accurately calculating your Tm with our Tm calculator.

Phire Green Hot Start II DNA Polymerase is also available (F-124S or F-124L) for direct loading of PCR products on gel.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Concentration
50X
Fidelity (vs. Taq)
2 X
GC-Rich PCR Performance
Medium
Hot Start
Built-In Hot Start
No. of Reactions
200 Reactions
Overhang
Blunt
Polymerase
Phire Hot Start II DNA polymerase
Purity
DHPLC
Reaction Format
Standalone
Reaction Speed
Fast
Starting Material
DNA
Volume
50 μL
Product Line
Phire
Product Type
Hot Start DNA Polymerase
Sufficient For
200 Reactions
For Use With (Application)
PCR Amplification

Contents & Storage

• 1 x 200 μL Phire Hot Start II DNA Polymerase
• 2 x 1.5 mL 5X Phire Reaction Buffer (provides 1.5 mM MgCl2)
• 1 x 500 μL DMSO

Store at -20°C.