Phire Green Hot Start II DNA Polymerase

Catalog number: F124S

Thermo Scientific™  Related applications: PCR

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Thermo Scientific Phire Hot Start II DNA Polymerase is an enhanced PCR enzyme for routine and high throughput PCR applications. It is significantly faster, extremely robust, and also capable of amplifying long DNA fragments with high yields. These features are achieved through advanced protein engineering of the polymerase. It incorporates a unique double-stranded DNA binding domain which allows short extension times (10 to 15 s/kb), improves yields, and increases fidelity 2-fold compared to Taq DNA polymerase.

The hot start modification of Phire Hot Start II DNA Polymerase is based on the Affibody inactivation technology. This technology increases the specificity of the PCR reaction with no additional time required for initial activation of the enzyme.

Phire Green format is a combination of Phire Hot Start II DNA Polymerase and 5X Phire Green Buffer. The buffer includes a density reagent and two tracking dyes for direct loading of PCR products on a gel. The colored buffer does not interfere with enzyme performance and is compatible with downstream applications such as DNA sequencing, ligation and restriction digestion.


Quick hot start—No reactivation step
Fast enzyme—Amplify four times faster than with hot start Taq
Robust—Minimal reaction optimization due to high inhibitor tolerance
High yields—Abundant products due to high efficiency
Longer PCR products—Amplify significantly longer DNA fragments than with any hot start Taq
Direct loading on gel with Green Buffer


• Hot Start PCR
• Routine PCR
• Non-high fidelity PCR
• Fast PCR
• High throughput PCR
• Genotyping

Note:The optimal annealing temperature for Phire DNA Polymerases may differ significantly from that of Taq-based polymerases. For optimal results start by accurately calculating your Tm with our Tm calculator.
For Research Use Only. Not for use in diagnostic procedures.


Polymerase: Phire Hot Start II DNA polymerase
Hot Start: Built-In Hot Start
Fidelity (vs. Taq): 2 X
GC-Rich PCR Performance: Medium
Reaction Format: Standalone
Product Overhang: Blunt
Reaction Speed: Fast
Product Size: 200 reactions

Contents & storage


• Phire Hot Start II DNA Polymerase
• 5X Phire Green Reaction Buffer (provides 1.5 mM MgCl2 in the final 1X concentration)
Store at -20°C.


Manuals & protocols

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