Thermo Scientific™

Phire Green Hot Start II PCR Master Mix

Catalog number: F126S
Thermo Scientific™

Phire Green Hot Start II PCR Master Mix

Catalog number: F126S
Catalog Number
F126S
also known as F-126S
Unit Size
200 reactions
Price (USD)
261.00
Your Price:
Availability
***
Quantity
Catalog Number
F126L
also known as F-126L
Unit Size
1,000 reactions
Price (USD)
1,014.00
Your Price:
Availability
***
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
F126S
also known as F-126S
200 reactions
261.00
Your Price:
***
F126L
also known as F-126L
1,000 reactions
1,014.00
Your Price:
***
Product Overview
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Thermo Scientific Phire Green Hot Start II PCR Master Mix is convenient 2X mix designed to minimize the number of pipetting steps. The master mix contains Phire Hot Start II DNA Polymerase, nucleotides and optimized reaction buffer including MgCl2. Only template and primers need to be added to PCR reaction. The master mix also includes a density reagent and two tracking dyes for direct loading of PCR products on a gel. The colored buffer does not interfere with enzyme performance and is compatible with downstream applications such as DNA sequencing, ligation and restriction digestion.

Phire Hot Start II DNA Polymerase, inluded in the master mix, is an enhanced PCR enzyme for routine and high throughput PCR applications. It is significantly faster, extremely robust, and also capable of amplifying long DNA fragments with high yields. These features are achieved through advanced protein engineering of the polymerase. It incorporates a unique double-stranded DNA binding domain which allows short extension times (10 to 15 s/kb), improves yields, and increases fidelity 2-fold compared to Taq DNA polymerase.

Highlights

Quick hot start—No reactivation step
Fast enzyme—Amplify four times faster than with hot start Taq
Robust—Minimal reaction optimization due to high inhibitor tolerance
High yields—Abundant products due to high efficiency
Longer PCR products—Amplify significantly longer DNA fragments than with any hot start Taq
Direct loading on gel

Applications

• Hot Start PCR
• Routine PCR
• Non-high fidelity PCR
• Fast PCR
• High throughput PCR
• Genotyping

Note:The optimal annealing temperature for Phire DNA Polymerases may differ significantly from that of Taq-based polymerases. For optimal results start by accurately calculating your Tm with our Tm calculator.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Concentration
2X
Detection Method
Primer-Probe
Fidelity (vs. Taq)
2 X
GC-Rich PCR Performance
Medium
Hot Start
Built-In Hot Start
Overhang
Blunt
Polymerase
Phire Hot Start II DNA polymerase
Reaction Format
SuperMix or Master Mix
Reaction Speed
Fast
For Use With (Application)
PCR Amplification
Includes
2X Phire Green Hot Start II PCR Master Mix (provides 1.5 mM MgCl2 in the final 1X concentration); Water, nuclease free; DMSO
Product Line
Phire
Product Type
Hot Start PCR Master Mix
Quantity
200 x 50μL rxns

Contents & Storage

• 4 x 1.25 mL 2X Phire Green Hot Start II PCR Master Mix
• 1 x 0.5 mL DMSO
• 4 x 1.25 mL Water, nuclease free

Store at -20°C.

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