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Phire Plant Direct PCR Kit (without sampling tools)
Thermo Scientific Phire Plant Direct PCR Kit is designed to amplify DNA directly from plant samples. No DNA purification is required prior to PCR.
Catalog number F130WH
Price (USD)/ 500 reactions
292.65
Online exclusive
361.00 Save 68.35 (19%)
-
Price (USD)/ 500 reactions
292.65
Online exclusive
361.00 Save 68.35 (19%)
Phire Plant Direct PCR Kit (without sampling tools)
Catalog numberF130WH
Price (USD)/ 500 reactions
292.65
Online exclusive
361.00 Save 68.35 (19%)
-
Thermo Scientific Phire Plant Direct PCR Kit is designed to amplify DNA directly from plant samples. No DNA purification is required prior to PCR. The kit is based on Phire Hot Start II DNA Polymerase, a specially engineered enzyme with a DNA binding domain. The unique features of this DNA polymerase make it extremely robust and tolerant of many PCR inhibitors present in plant, fungi, and bacteria.
The Phire Plant Direct PCR Kit includes a complete set of optimized PCR reagents as well as detailed protocols for different sample types, making it an ideal choice for amplification of plant DNA. In addition, the kit includes control primers for positive control reactions. The control primers amplify a highly conserved region of chloroplast DNA and are universal for many plant species.
Features
- No need for time-consuming and expensive DNA purification steps
- Very little sample material required
- Two simple protocols for various applications
- Phire Hot Start II DNA Polymerase provides high yields of specific product with short extension time (20 s/kb)
Applications
- Genotyping
- Transgene detection
Notes
- The optimal annealing temperature for Phire DNA Polymerase may differ significantly from that of Taq-based polymerases.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
GC-Rich PCR PerformanceMedium
PCR MethodDirect PCR
PolymerasePhire Hot Start II DNA Polymerase
Reaction SpeedFast
Product TypeDirect PCR Kit
Shipping ConditionWet or Dry Ice
Hot StartBuilt-In Hot Start
No. of Reactions500 Reactions
OverhangBlunt
Reaction FormatSeparate Components
Sample TypePlant
Starting MaterialDNA
Unit Size500 reactions
Contents & Storage
• Phire Hot Start II DNA Polymerase, 200 μL
• 2X Phire Plant Tissue PCR Buffer, 5 x 1.25 mL
• Universal Control Primer Mix (25 μM each), 40 μL
• Dilution Buffer, 2 x 5 mL
Store at -20°C.
• 2X Phire Plant Tissue PCR Buffer, 5 x 1.25 mL
• Universal Control Primer Mix (25 μM each), 40 μL
• Dilution Buffer, 2 x 5 mL
Store at -20°C.