Phire Animal Tissue Direct PCR Kit (without sampling tools)

Thermo Scientific™

Phire Animal Tissue Direct PCR Kit (without sampling tools)

Thermo Scientific Phire Animal Tissue Direct PCR Kit has been developed for amplification of DNA directly from a wide variety of animal tissues including mice, fish, birds and insects.

카탈로그 번호	수량
F140WH	500 reactions

카탈로그 번호 F140WH

제품 가격(KRW)

531,000

온라인 행사

Ends: 31-Mar-2026

606,000

할인액 75,000 (12%)

카트에 추가하기

500 reactions

대량 주문 또는 맞춤형 요청

제품 가격(KRW)

531,000

온라인 행사

Ends: 31-Mar-2026

606,000

할인액 75,000 (12%)

카트에 추가하기

Thermo Scientific Phire Animal Tissue Direct PCR Kit has been developed for amplification of DNA directly from a wide variety of animal tissues including mice, fish, birds, and insects. The kit contains all the necessary components for amplification of DNA directly from animal tissues: optimized PCR reagents, dilution buffer, and DNARelease additive, which can be used to improve the release of DNA from animal tissues. In addition, the kit includes control primers for positive control reactions that are universal and work with numerous animal species.

Features

No need for time-consuming and expensive DNA purification steps
Very little sample material required
Two simple protocols for various applications
Phire Hot Start II DNA Polymerase provides high yields of specific product with short extension time (20 s/kb).

Notes

A detailed manual is provided that describes protocols when working with different types of tissues.
The optimal annealing temperature for Phire DNA Polymerase may differ significantly from that of Taq-based polymerases.

For Research Use Only. Not for use in diagnostic procedures.

핫 스타트Built-In Hot Start

반응 수500 reactions

오버행Blunt

중합효소Phire Hot Start II DNA Polymerase

제품 유형Direct PCR Kit

수량500 reactions

반응 형식Separate Components

샘플 종류Tissue

배송 조건Wet or Dry Ice

시작 물질DNA

GC-Rich PCR PerformanceMedium

PCR 방법Direct PCR

반응 속도Fast

Unit SizeEach

구성 및 보관

• Phire Hot Start II DNA Polymerase, 200 μL
• 2X Phire Animal Tissue PCR Buffer, 5 x 1.25 mL
• Control Primer Mix (25 μM each), 40 μL
• Dilution Buffer, 2 x 5 mL
• DNARelease Additive, 3 x 100 μL
• Gel Loading Dye, 3 x 1 mL

Store at -20°C.

자주 묻는 질문(FAQ)

What is your recommendation to take a sample from plant or animal tissue for Direct PCR?

In order to obtain best results from Direct PCR, it is important to use a very small amount of starting materials. The recommended size is 0.3-0.5 mm in diameter. We recommend using Tissue Puncher 0.3 mm (Cat. No. F-200S), which is a convenient tool for sampling and is suitable for both plant and animal tissues.

Why are Control primers provided in the Direct PCR Kits?

It is very important to include a control reaction in the Direct PCR to ensure that the PCR conditions are optimal for the specific tissue or plant material used. Control primers are universal primers that amplify a small fragment from a conserved genome region. If the reaction with control primers fails, the sample material may not be suitable for Direct PCR, or PCR conditions need optimization.

How are samples, stored at different conditions, compatible with the Phire Tissue Direct PCR Master Mix?

With the Phire Tissue Direct PCR Master Mix you can amplify DNA from differently stored tissues (fresh, frozen, FFPE) from human and a variety of animal species.

Can I use plant or tissue sample which is not on the list of the Direct PCR Master Mix and Kit tested samples?

You can test any plant or tissue sample. However, it is recommended to first optimize the reaction. When working with new sample materials, start with the Dilution & Storage protocol as it allows several PCR reactions to be performed from the same sample during optimization. It is recommended to perform a positive control with purified sample DNA to ensure that the PCR conditions are working. If the positive control with purified DNA fails, the PCR conditions should be optimized before continuing further.

Can you amplify the same sample materials with the Direct PCR Master Mixes as with Direct PCR Kits?

Yes, the Direct PCR Master Mixes are upgrades of the Direct PCR kits and thus are suitable for the same sample materials.

View all Phire Animal Tissue Direct PCR Kit (without sampling tools) FAQs