Phire Animal Tissue Direct PCR Kit (without sampling tools)
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Phire Animal Tissue Direct PCR Kit (without sampling tools)
Thermo Scientific™

Phire Animal Tissue Direct PCR Kit (without sampling tools)

Thermo Scientific Phire Animal Tissue Direct PCR Kit has been developed for amplification of DNA directly from a wide variety of animal tissues including mice, fish, birds and insects.
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Catalog number F140WH
Price (USD)/ 500 reactions
405.65
Online exclusive
474.00 
Save 68.35 (14%)
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Add to cart
Request bulk or custom format
Price (USD)/ 500 reactions
405.65
Online exclusive
474.00 
Save 68.35 (14%)
Add to cart
Phire Animal Tissue Direct PCR Kit (without sampling tools)
Catalog numberF140WH
Price (USD)/ 500 reactions
405.65
Online exclusive
474.00 
Save 68.35 (14%)
-
Add to cart
Thermo Scientific Phire Animal Tissue Direct PCR Kit has been developed for amplification of DNA directly from a wide variety of animal tissues including mice, fish, birds, and insects. The kit contains all the necessary components for amplification of DNA directly from animal tissues: optimized PCR reagents, dilution buffer, and DNARelease additive, which can be used to improve the release of DNA from animal tissues. In addition, the kit includes control primers for positive control reactions that are universal and work with numerous animal species.

Features

  • No need for time-consuming and expensive DNA purification steps
  • Very little sample material required
  • Two simple protocols for various applications
  • Phire Hot Start II DNA Polymerase provides high yields of specific product with short extension time (20 s/kb).

Notes

  • A detailed manual is provided that describes protocols when working with different types of tissues.
  • The optimal annealing temperature for Phire DNA Polymerase may differ significantly from that of Taq-based polymerases.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
GC-Rich PCR PerformanceMedium
PCR MethodDirect PCR
PolymerasePhire Hot Start II DNA Polymerase
Reaction SpeedFast
Product TypeDirect PCR Kit
Shipping ConditionWet or Dry Ice
Hot StartBuilt-In Hot Start
No. of Reactions500 reactions
OverhangBlunt
Reaction FormatSeparate Components
Sample TypeTissue
Starting MaterialDNA
Unit Size500 reactions
Contents & Storage
• Phire Hot Start II DNA Polymerase, 200 μL
• 2X Phire Animal Tissue PCR Buffer, 5 x 1.25 mL
• Control Primer Mix (25 μM each), 40 μL
• Dilution Buffer, 2 x 5 mL
• DNARelease Additive, 3 x 100 μL
• Gel Loading Dye, 3 x 1 mL

Store at -20°C.