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Phusion Human Specimen Direct PCR Kit
Thermo Scientific Phusion Human Specimen Direct PCR Kit delivers unprecedented convenience for DNA amplification by allowing PCR directly from unpurified human samples.
Catalog number F150BID
Price (USD)/ 200 reactions
272.65
Online exclusive
349.00 Save 76.35 (22%)
-
Price (USD)/ 200 reactions
272.65
Online exclusive
349.00 Save 76.35 (22%)
Phusion Human Specimen Direct PCR Kit
Catalog numberF150BID
Price (USD)/ 200 reactions
272.65
Online exclusive
349.00 Save 76.35 (22%)
-
Thermo Scientific Phusion Human Specimen Direct PCR Kit delivers unprecedented convenience for DNA amplification by allowing PCR directly from unpurified human samples. A tiny amount of sample is used directly in the PCR reaction with no prior purification steps. This allows significant savings in both time and cost.
Tested sample materials include buccal swabs, saliva, amniotic fluid, hair, fingernails, teeth, and skin biopsies. The kit is compatible with fresh, frozen, and even formalin-fixed, paraffin-embedded (FFPE) human tissue samples. The Phusion Human Specimen Direct PCR Kit includes optimized reagents and detailed protocols for many sample materials. The modified Phusion Hot Start II High-Fidelity DNA Polymerase utilized in the kit is a highly accurate proofreading DNA polymerase, and PCR products obtained are perfectly suitable even for cloning purposes.
Features
- No need for time-consuming and expensive DNA purification steps
- Two simple protocols for various applications
- Extremely short PCR protocol times
- Modified Phusion Hot Start II High-Fidelity DNA Polymerase delivers abundant yields of specific product
Notes
- The optimal annealing temperature for Phusion DNA Polymerases may differ significantly from that of Taq-based DNA polymerases.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
GC-Rich PCR PerformanceHigh
PCR MethodDirect PCR
PolymerasePhusion Human Specimen DNA Polymerase
Reaction SpeedFast
Product TypeDirect PCR Kit
Shipping ConditionWet or Dry Ice
For Use With (Application)Hot-start PCR
Hot StartBuilt-In Hot Start
No. of Reactions200 reactions
OverhangBlunt
Reaction FormatSeparate Components
Sample TypeHuman
Starting MaterialDNA
Unit Size200 reactions
Contents & Storage
• Phusion Human Specimen DNA Polymerase, 80 μL
• 2X Phusion Human Specimen PCR Buffer, 2 x 1.25 mL
• Universal Control Primer Mix (25 μM), 40 μL
• Dilution Buffer, 2 x 5 mL
• DNARelease Additive, 3 x 100 μL
Store at -20°C.
• 2X Phusion Human Specimen PCR Buffer, 2 x 1.25 mL
• Universal Control Primer Mix (25 μM), 40 μL
• Dilution Buffer, 2 x 5 mL
• DNARelease Additive, 3 x 100 μL
Store at -20°C.