Phusion Human Specimen Direct PCR Kit

Catalog number: F150BID

Thermo Scientific™  Related applications: PCR

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Thermo Scientific Phusion Human Specimen Direct PCR Kit delivers unprecedented convenience for DNA amplification by allowing PCR directly from unpurified human samples. A tiny amount of sample is used directly in the PCR reaction with no prior purification steps. This allows significant savings in both time and cost.

Tested sample materials include buccal swabs, saliva, amniotic fluid, hair, fingernails, teeth, and skin biopsies. The kit is compatible with fresh, frozen, and even Formalin-Fixed, Paraffin Embedded (FFPE) human tissue samples. It includes optimized reagents and detailed protocols for many sample materials. The modified Phusion Hot Start II High-Fidelity DNA Polymerase utilized in the kit is a highly accurate proofreading DNA polymerase; PCR products obtained are perfectly suitable even for cloning purposes.


• No need for time-consuming and expensive DNA purification steps
• Two simple protocols for various applications
• Extremely short PCR protocol times
• Modified Phusion Hot Start II High-Fidelity DNA Polymerase delivers abundant yields of specific product

The optimal annealing temperature for Phusion DNA Polymerases may differ significantly from that of Taq-based DNA polymerases.

For optimal results start by accurately calculating your Tm with our Tm calculator.
For Research Use Only. Not for use in diagnostic procedures.


Polymerase: Phusion Human Specimen DNA Polymerase
Hot Start: Built-In Hot Start
GC-Rich PCR Performance: High
Reaction Format: Separate Components
Product Overhang: Blunt
Reaction Speed: Fast
Product Size: 200 reactions

Contents & storage

• 80 µL Phusion Human Specimen DNA Polymerase
• 2 x 1.25 mL 2X Phusion Human Specimen PCR Buffer (includes dNTPs and MgCl2)
• 40 µL Universal Control Primer Mix (25µM each)
• 2 x 5 mL Dilution Buffer
• 3 x 100 µL DNARelease Additive

Store at -20°C.


Manuals & protocols