Phire Plant Direct PCR Master Mix
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Phire Plant Direct PCR Master Mix
Thermo Scientific™

Phire Plant Direct PCR Master Mix

Thermo Scientific Phire Plant Direct PCR Master Mix is designed to amplify DNA directly from plant samples.
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Catalog NumberNo. of Reactions
F160L1250 Reactions
F160S250 Reactions
Catalog number F160L
Price (USD)/ 1,250 reactions
589.65
Online exclusive
800.00 
Save 210.35 (26%)
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Add to cart
No. of Reactions:
1250 Reactions
Request bulk or custom format
Price (USD)/ 1,250 reactions
589.65
Online exclusive
800.00 
Save 210.35 (26%)
Add to cart
Phire Plant Direct PCR Master Mix
Catalog numberF160L
Price (USD)/ 1,250 reactions
589.65
Online exclusive
800.00 
Save 210.35 (26%)
-
Add to cart
Thermo Scientific Phire Plant Direct PCR Master Mix is designed to amplify DNA directly from plant samples. No DNA purification is required prior to PCR. The kit is based on Phire Hot Start II DNA Polymerase, a specially engineered enzyme with a DNA binding domain. The unique features of this DNA polymerase make it extremely robust and tolerant of many PCR inhibitors present in plant material like complex polysaccharides, polyphenols, and others.

Phire Plant Direct PCR Master Mix has been tested with leaves and seeds from a wide variety of plant species as well as fungi, bacteria, and yeast. The kit includes a complete set of optimized reagents and detailed protocols, making it an ideal choice for amplification of plant DNA.

Features

  • Direct PCR allows sample to be added directly to PCR reaction, reducing time-consuming and expensive DNA purification steps
  • Available Dilution & Storage protocol allows several PCR reactions from one sample
  • Specially engineered Phire Hot Start II DNA Polymerase for extremely short PCR protocol times
  • Master mix format with premixed gel loading dye minimizes risk of cross-contamination, reduces sample handling, and allows direct loading on gel

Applications

  • Genotyping
  • Transgene detection
  • Knockout analysis
  • Sequencing

Notes

  • Annealing temperature for Phire DNA Polymerases may differ significantly from that of Taq-based polymerases.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
GC-Rich PCR PerformanceMedium
PCR MethodDirect PCR
PolymerasePhire Hot Start II DNA Polymerase
Reaction SpeedFast
Product TypeDirect PCR Master Mix
Shipping ConditionWet or Dry Ice
For Use With (Application)Genotyping, Hot-start PCR
Concentration2X
Hot StartBuilt-In Hot Start
No. of Reactions1250 Reactions
OverhangBlunt
Reaction FormatSuperMix or Master Mix
Sample TypePlant
Starting MaterialDNA
Unit Size1,250 reactions
Contents & Storage
• 2X Phire Plant Direct PCR Master Mix, 10 x 1.25 mL
• Dilution Buffer, 2 x 5 mL
• 25 μM Control Primer Mix, 40 μL
• Nuclease-free water, 10 x 1.25 mL
• O'GeneRuler Express DNA Ladder, 50 μg (sufficient for 100 applications)

Store at -20°C.