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Thermo Scientific™

Phire Plant Direct PCR Master Mix

Catalog number: F160S
Thermo Scientific™

Phire Plant Direct PCR Master Mix

Catalog number: F160S
Catalog Number
F160S
also known as F-160S
Unit Size
250 reactions
Price (USD)
Availability
-
Quantity
Catalog Number
Unit Size
1,250 reactions
Price (USD)
Availability
-
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
F160S
also known as F-160S
250 reactions
-
F160L1,250 reactions
-
Product Overview
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Thermo Scientific Phire Plant Direct PCR Master Mix is designed to amplify DNA directly from plant samples. No DNA purification is required prior to PCR. The kit is based on Phire Hot Start II DNA Polymerase, a specially engineered enzyme with a DNA binding domain. The unique features of this DNA polymerase make it extremely robust and tolerant of many PCR inhibitors present in plant material like complex polysaccharides, polyphenols and others.

Phire Plant Direct PCR Master Mix has been tested with leaves and seeds from a wide variety of plant species as well as fungi, bacteria and yeast. The kit includes a complete set of optimized reagents and detailed protocols making it an ideal choice for amplification of plant DNA.

Highlights

• Direct PCR—sample is added directly to PCR reaction, therefore there is no need for time-consuming and expensive DNA purification steps.
• Dilution & Storage protocol available—allows tens of PCR reactions from one tiny sample and allows re-testing
• Specially engineered Phire Hot Start II DNA polymerase—extremely short PCR protocol times
• Master Mix format with premixed gel loading dye—minimizes possibility of cross-contamination, reduces sample handling and allows direct loading on gel

Applications

• Genotyping
• Transgene detection
• Knockout analysis
• Sequencing

The optimal annealing temperature for Phire DNA Polymerases may differ significantly from that of Taq-based polymerases.

For optimal results start by accurately calculating your Tm with our Tm calculator.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

GC-Rich PCR Performance
Medium
PCR Method
Direct PCR
Polymerase
Phire Hot Start II DNA polymerase
Reaction Speed
Fast
Includes Label or Dye
Yes
Product Line
Phire
Product Type
Direct PCR Master Mix
Quantity
250 x 50 μL Reactions
For Use With (Application)
Genotyping
Hot Start
Built-In Hot Start
No. of Reactions
250 x 50 L Reactions
Overhang
Blunt
Reaction Format
SuperMix or Master Mix
Sample Type
Plant
Starting Material
DNA

Contents & Storage

Contains:
• 2 x 1.25 mL Phire Plant Direct PCR Master Mix (2X)
• 5 mL Dilution Buffer
• 40 μL Control Primer Mix (25 μM each)
• 2 x 1.25 mL Water, nuclease- free
• 50 μg O'GeneRuler Express DNA Ladder (sufficient for 100 applications)

Store at -20°C.

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