Thermo Scientific™

Phire Tissue Direct PCR Master Mix

Catalog number: F170S
Thermo Scientific™

Phire Tissue Direct PCR Master Mix

Catalog number: F170S
Catalog Number
F170S
also known as F-170S
Unit Size
250 reactions
Price (USD)
208.00
Your Price:
Availability
***
Quantity
Catalog Number
F170L
also known as F-170L
Unit Size
1,250 reactions
Price (USD)
880.00
Your Price:
Availability
***
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
F170S
also known as F-170S
250 reactions
208.00
Your Price:
***
F170L
also known as F-170L
1,250 reactions
880.00
Your Price:
***
Product Overview
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Additional Information
Recommendations
Recommendations
Thermo Scientific Phire Tissue Direct PCR Master Mix has been developed for amplification of DNA directly from a wide variety of tissues obtained from mice, human, fish, birds and insects. The Master Mix containing Phire Hot Start II DNA Polymerase is specially formulated to perform PCR in the presence of different animal tissue-derived inhibitors such as collagen, melanin and eumelanin (hair, skin) or myoglobin (muscle). The kit also includes Thermo Scientific DNARelease Additive, which can be used to improve the release of DNA from difficult tissues.

Highlights

• Direct PCR—sample is added directly to PCR reaction, therefore there is no need for time-consuming and expensive DNA purification steps
• Dilution & Storage protocol available—allows tens of PCR reactions from one tiny sample and allows re-testing
• Specially engineered Phire DNA polymerase—extremely short PCR protocol times
• Master Mix format with premixed gel loading dye—minimizes possibility of cross-contamination, reduces sample handling and allows direct loading on gel

Applications

• Genotyping
• Transgene detection
• Knockout analysis
• Sequencing

The optimal annealing temperature for Phire DNA Polymerases may differ significantly from that of Taq-based polymerases.

For optimal results start by accurately calculating your Tm with our Tm calculator.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

GC-Rich PCR Performance
Medium
Hot Start
Built-In Hot Start
No. of Reactions
250 Reactions
Overhang
Blunt
PCR Method
Direct PCR
Polymerase
Phire Hot Start II DNA polymerase
Reaction Format
Master Mix
Reaction Speed
Fast
Sample Type
Tissue
Starting Material
DNA
For Use With (Application)
Direct PCR
Includes Label or Dye
Yes
Product Line
Phire
Product Type
Direct PCR Master Mix
Quantity
250 x 20μL Reactions
Sufficient For
250 x 20 μL Reactions

Contents & Storage

Contains:
• 2 x 1.25 mL Phire Tissue Direct PCR Master Mix (2X)
• 5 mL Dilution Buffer
• 3 x 100 µL DNARelease Additive
• 40 µL Control Primer Mix (25 µM each)
• 2 x 1.25 mL Water, nuclease- free
• 50 µg O'GeneRuler Express DNA Ladder (sufficient for 100 applications)

Store at -20°C.

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