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Phusion Blood Direct PCR Master Mix
Thermo Scientific Phusion Blood Direct PCR Master Mix is designed for amplification of DNA from whole blood. It eliminates the need for a separate DNA purification step prior to PCR.
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| Catalog Number | No. of Reactions |
|---|---|
| F175L | 500 Reactions |
| F175S | 100 Reactions |
Catalog number F175L
Price (USD)/ 500 reactions
570.00
-
No. of Reactions:
500 Reactions
Price (USD)/ 500 reactions
570.00
Phusion Blood Direct PCR Master Mix
Catalog numberF175L
Price (USD)/ 500 reactions
570.00
-
Thermo Scientific Phusion Blood Direct PCR Master Mix is designed for amplification of DNA from whole blood. It eliminates the need for a separate DNA purification step prior to PCR. The modified Phusion Hot Start II High-Fidelity DNA Polymerase is resistant to main PCR inhibitors present in blood and retains its activity at blood concentrations of up to 40% in the PCR reaction.
This accurate proofreading DNA polymerase makes the Phusion Blood Direct PCR Master Mix an ideal choice for demanding applications, such as cloning. Phusion Blood Direct PCR Master Mix also allows analysis of blood samples with different preservatives (e.g., heparin, EDTA, citrate), storage conditions, and from various species.
Highlights
- Direct PCR allows sample to be added directly to PCR reaction, reducing time-consuming and expensive DNA purification steps
- Available Dilution & Storage protocol allows several PCR reactions from one sample
- Specially engineered Phire Hot Start II DNA polymerase for extremely short PCR protocol times
- Master mix format with premixed gel loading dye minimizes risk of cross-contamination, reduces sample handling, and allows direct loading on gel
Applications
- Genotyping
- Transgene detection
- Knockout analysis
- Sequencing
- Cloning
Notes
- Annealing temperature for Phire DNA Polymerases may differ significantly from that of Taq-based polymerases.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
GC-Rich PCR PerformanceHigh
PCR MethodDirect PCR
PolymerasePhusion Blood II DNA Polymerase
Reaction SpeedFast
Includes Label or DyeYes
Product TypeDirect PCR Master Mix
Shipping ConditionWet or Dry Ice
For Use With (Application)Genotyping
Concentration2X
Hot StartBuilt-In Hot Start
No. of Reactions500 Reactions
OverhangBlunt
Reaction FormatSuperMix or Master Mix
Sample TypeBlood
Starting MaterialDNA
Unit Size500 reactions
Contents & Storage
• 2X Phusion Blood Direct PCR Master Mix, 5 x 1 mL
• Universal Control Primer Mix, 40 μL
• 50 mM EDTA, 0.5 mL
• 50 mM DMSO, 0.5 mL
• 50 mM MgCl2, 1.5 mL
• Nuclease-free water, 4 x 1.25 mL
• O'GeneRuler Express DNA Ladder, 50 μg (sufficient for 100 applications)
Store at -20°C.
• Universal Control Primer Mix, 40 μL
• 50 mM EDTA, 0.5 mL
• 50 mM DMSO, 0.5 mL
• 50 mM MgCl2, 1.5 mL
• Nuclease-free water, 4 x 1.25 mL
• O'GeneRuler Express DNA Ladder, 50 μg (sufficient for 100 applications)
Store at -20°C.