A reevaluation of neuronal zinc measurements: artifacts associated with high intracellular dye concentration.
AuthorsDineley KE, Malaiyandi LM, Reynolds IJ
JournalMol Pharmacol
PubMed ID12181438
The emergence of zinc as a potent neurotoxin has prompted the development of techniques suitable for the measurement of intracellular free zinc ([Zn(2+)](i)) in cultured cells. Accordingly, a new family of Zn(2+)-sensitive fluorophores has become available. Using ionophore-induced elevations of [Zn(2+)](i) in cultured neurons, we measured [Zn(2+)](i)-induced changes in the ... More
Kinetic study of the antiport mechanism of an Escherichia coli zinc transporter, ZitB.
AuthorsChao Y, Fu D
JournalJ Biol Chem
PubMed ID14715669
ZitB is a member of the cation diffusion facilitator (CDF) family that mediates efflux of zinc across the plasma membrane of Escherichia coli. We describe the first kinetic study of the purified and reconstituted ZitB by stopped-flow measurements of transmembrane fluxes of metal ions using a metal-sensitive fluorescent indicator encapsulated ... More