Fluorocillin™ Green 495/525 -Lactamase Substrate, soluble product

Catalog number:  F33952

Invitrogen™  Related applications: Cellular Imaging | Protein Assays and Analysis

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Fluorocillin™ Green substrate is a robust reporter for ELISA protocols that employ TEM-1 β-lactamase conjugates. Upon cleavage, Fluorocillin™ Green reagent is converted to a green-fluorescent soluble product (emission maxima at ~525 nm). Fluorocillin™ Green reagent has a broad dynamic range of fluorescence signal, is more sensitive than common colorimetric substrates, and displays only modest hydrolysis when incubated for extended periods from pH 5.5 to 8.0. Additionally, Fluorocillin™ Green reagent consistently reports β-lactamase activity in the presence of EDTA, many detergents, salts, and sodium azide. Fluorocillin™ Green reagent is available as a dry powder, packaged in five vials each containing 100 µg (F33952) and as a component of the SensiFlex™ ELISA Development Kits (S33853, S33854).
For Research Use Only. Not for use in diagnostic procedures.


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Detection Method: Fluorescent
Product Line: Fluorocillin™
Product Size: 5 x 100 µg
Substrate: Fluorocillin™ Green 495⁄525
Substrate Properties: Chemical Substrate
Substrate Type: Beta-Lactamase Substrate
Shipping Condition: Room Temperature

Contents & storage

Store in freezer (-5 to -30°C) and protect from light.


Manuals & protocols

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