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Thermo Scientific™

Phusion High-Fidelity PCR Master Mix with HF Buffer

Catalog number: F531S
Thermo Scientific™

Phusion High-Fidelity PCR Master Mix with HF Buffer

Catalog number: F531S
Catalog Number
F531S
also known as F-531S
Unit Size
100 reactions
Price (USD)
Price: 222.00
Online price: 135.65
Your price:
Availability
-
Quantity
Catalog Number
Unit Size
500 reactions
Price (USD)
Price: 882.00
Online price: 697.65
Your price:
Availability
-
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
F531S
also known as F-531S
100 reactions
Price: 222.00
Online price: 135.65
Your price:
-
F531L500 reactions
Price: 882.00
Online price: 697.65
Your price:
-
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Thermo Scientific Phusion High-Fidelity DNA Polymerases set a gold standard for high performance PCR. Featuring an error rate 50-fold lower than that of Taq, and 6-fold lower than that of Pfu, Phusion High-Fidelity DNA Polymerase is excellent choice for cloning and other applications requiring high fidelity. Phusion DNA Polymerases offer robust performance with short protocol times, even in the presence of PCR inhibitors, and generate higher yields with lower enzyme amounts than other DNA polymerase.

Phusion High-Fidelity PCR Master Mix is convenient 2X mix containing Phusion DNA Polymerase, nucleotides and optimized reaction buffer including MgCl2. Two master mix formulations are available - with HF Buffer (F-531S and F-531L) and with GC Buffer (F-532S and F-532L). The error rate of Phusion DNA Polymerase in HF Buffer (4.4 x 10-7) is lower than that in GC Buffer (9.5 x 10-7). Therefore, the master mix with HF Buffer should be used as the default master mix for high-fidelity amplification. However, GC Buffer can improve the performance of Phusion DNA Polymerase on some difficult or long templates, such as GC-rich templates or those with complex secondary structures.

Highlights
• High fidelity (50X Taq)
• Fast PCR due to short extension times (15-30 s/kb)
• Robust performance, minimal optimization needed

Applications
• High-fidelity PCR
• Amplification of difficult (GC-rich) templates
• Cloning
• Template generation for sequencing
• Long-range PCR (up to 20 kb)
• Mutagenesis
• High throughput PCR

Using Phusion DNA Polymerases
Annealing rules for Phusion DNA Polymerases are different from many common DNA polymerases (such as Taq DNA polymerases). For optimal results, use our Tm calculator at www.thermofisher.com/tmcalculator.

For Research Use Only. Not for use in diagnostic procedures.

Specifications

GC-Rich PCR Performance
Medium
Polymerase
Phusion High-Fidelity DNA Polymerase
Reaction Speed
Fast
Product Type
High-Fidelity PCR Master Mix
Shipping Condition
Dry Ice
For Use With (Application)
Standard PCR, High-fidelity PCR
Concentration
2X
Fidelity (vs. Taq)
52X
Hot Start
No
No. of Reactions
100 Reactions
Overhang
Blunt
Reaction Format
SuperMix or Master Mix
Size (Final Product)
20 kb or less

Contents & Storage

• 2X Phusion Master Mix with HF Buffer (2 x 1.25 mL)
• 100% DMSO (1 x 500 μL)

Store at –20°C.

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