Phusion U Hot Start PCR Master Mix

Catalog number: F533L

Thermo Scientific™  Related applications: PCR

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Thermo Scientific Phusion U DNA polymerase is a novel engineered high fidelity enzyme developed using fusion technology. Due to a proprietary mutation in the so called dUTP binding pocket of Phusion, Phusion U overcomes an important limitation of proofreading enzymes - it is able to incorporate dUTP and read through uracil present in DNA templates.

In addition to uracil usage possibility, Phusion U features all the superior properties of other Phusion DNA Polymerases - great accuracy, speed, ability to amplify long amplicons up to 20 kb and a high specificity with Affibody based hot start. These features make Phusion U Hot Start DNA Polymerase an excellent choice for such important applications as amplification of bisulphite-converted or damaged DNA as well as use of carryover contamination control.

Phusion U Hot Start PCR Master Mix is convenient 2X mix containing Phusion U Hot Start DNA Polymerase, nucleotides and optimized reaction buffer including MgCl2. Only template and primers need to be added by the user.


Accuracy—high fidelity DNA polymerase (25x Taq)
Uracil-tolerance —engineered to incorporate dUTP and amplify uracil containing templates
Specificity—hot start for reduced nonspecific amplification and primer degradation
Speed —short extension times (15-30 s/kb)


• Amplification of bisulphite-converted DNA
• Amplification of damaged or aged DNA
• Carry-over contamination control
• Uracil-excision based (USER) cloning methods
For Research Use Only. Not for use in diagnostic procedures.


Polymerase: Phusion U Hot Start DNA Polymerase
Hot Start: Built-In Hot Start
Fidelity (vs. Taq): 25 X
GC-Rich PCR Performance: Medium
Reaction Format: SuperMix or Master Mix
Product Overhang: Blunt
Reaction Speed: Fast
Product Size: 500 reactions

Contents & storage

Phusion U Hot Start PCR Master Mix includes:
• Phusion U Hot Start DNA Polymerase
• 2X reaction buffer
• 3 mM MgCl2
• 400 µM of each dATP, dTTP, dCTP and dGTP
The master mix is supplied with nuclease-free water.
Store at -20°C.


Manuals & protocols

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