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Phusion Blood Direct PCR Kit
Thermo Scientific Phusion Blood Direct PCR Kit is developed for amplification of DNA from whole blood. It eliminates the need for a separate DNA purification step prior to PCR.
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| Catalog Number | No. of Reactions |
|---|---|
| F547L | 500 Reactions |
| F547S | 100 Reactions |
Catalog number F547L
Price (USD)/ 500 reactions
417.65
Online exclusive
600.00 Save 182.35 (30%)
-
No. of Reactions:
500 Reactions
Price (USD)/ 500 reactions
417.65
Online exclusive
600.00 Save 182.35 (30%)
Phusion Blood Direct PCR Kit
Catalog numberF547L
Price (USD)/ 500 reactions
417.65
Online exclusive
600.00 Save 182.35 (30%)
-
Thermo Scientific Phusion Blood Direct PCR Kit is for use in the amplification of DNA from whole blood. It eliminates the need for a separate DNA purification step prior to PCR. The modified Phusion Hot Start II High-Fidelity DNA Polymerase exhibits high resistance to PCR inhibitors found in blood and retains polymerase activity even with samples containing 40% whole blood.
This DNA polymerase exhibits exceptional accuracy in proofreading and makes the Phusion Blood Direct PCR Kit one of the best choices for demanding applications, such as cloning. The Phusion Blood Direct PCR Kit is versatile and permits analysis of blood samples from various species and with different anticoagulants or storage conditions.
Features
- No need for time-consuming and expensive DNA purification steps
- Simple protocol requires minimal hands-on time
- Extremely short PCR protocol times
- Modified Phusion Hot Start II High-Fidelity DNA Polymerase delivers abundant yields of specific product
Notes
- The optimal annealing temperature for Phusion DNA Polymerases may differ significantly from that of Taq-based polymerases.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
GC-Rich PCR PerformanceHigh
PCR MethodDirect PCR
PolymerasePhusion Blood II DNA Polymerase
Reaction SpeedFast
Product TypeDirect PCR Kit
Shipping ConditionWet or Dry Ice
Concentration2 U/μL
Hot StartBuilt-In Hot Start
No. of Reactions500 Reactions
OverhangBlunt
Reaction FormatSeparate Components
Sample TypeBlood
Starting MaterialDNA
Unit Size500 reactions
Contents & Storage
• Phusion Blood II DNA Polymerase, 200 μL
• 2X Phusion Blood PCR Buffer with dNTPs and MgCl2, 5 x 1.25 mL
• Universal Control Primer Mix, 40 μL
• 50 mM EDTA, 0.5 mL
• 50 mM MgCl2, 1.5 mL
• DMSO, 0.5 mL
Store at -20°C.
• 2X Phusion Blood PCR Buffer with dNTPs and MgCl2, 5 x 1.25 mL
• Universal Control Primer Mix, 40 μL
• 50 mM EDTA, 0.5 mL
• 50 mM MgCl2, 1.5 mL
• DMSO, 0.5 mL
Store at -20°C.