Thermo Scientific™

Phusion Flash High-Fidelity PCR Master Mix

Catalog number:  F548S

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Thermo Scientific Phusion Flash PCR Master Mix was developed to save valuable laboratory time. Ready-to-use 2X master mix preserves the fidelity and the yield in the reaction when using extremely short PCR protocols. Additionally, the user only needs to add template and primers minimizing the number of pipetting steps.

The unique master mix composition permits usage of extremely short cycling protocols with both low and high complexity DNA templates - 15 seconds per kilobase or less. The master mix utilizes Phusion Flash II DNA Polymerase, a modified proofreading DNA polymerase derived from Phusion Hot Start II High-Fidelity DNA Polymerase.

• Extreme speed: extension times of 15 s/kb or less
• Hot start modification allowing “zero-time reactivation”
• Accuracy: proofreading DNA polymerase with a fidelity of 25X Taq
• High yields in reduced time


• Fast PCR
• High-fidelity PCR
• Difficult (GC-rich) templates
• Template generation for sequencing
• Multiplex PCR
• Long-range PCR
• Cloning
• Mutagenesis
• Microarray

Using Phusion DNA Polymerases
Annealing rules for Phusion DNA Polymerases are different from many common DNA polymerases (such as Taq DNA polymerases). For optimal results, use our Tm calculator at

For Research Use Only. Not for use in diagnostic procedures.


Polymerase: Phusion Flash II DNA Polymerase
Hot Start: Built-In Hot Start
Fidelity (vs. Taq): 25 X
GC-Rich PCR Performance: High
Reaction Format: SuperMix or Master Mix
Product Overhang: Blunt
Reaction Speed: Fast
Product Line: Phusion
Product Size: 100 reactions

Contents & storage

Phusion Flash High-Fidelity PCR Master Mix contains Phusion Flash II DNA Polymerase, 2X reaction buffer, dNTPs, and MgCl2. It is sufficient for 100 reactions in 20 µL volume.
Store at -20°C.


Manuals & protocols